石蜡包埋组织DNA检测在判断畸形儿病因中的应用  

Detecting Pathogens of Fetal Defects in Paraffin Embedded Tissues by Polymerase Chain Reaction

作　　者：刘伯宁[1] 孙剑英[1] 陶雯琪[1] 刘德莉[1] 张剑麟[1] 

机构地区：[1]上海市第六人民医院妇产病理研究室

出　　处：《中华妇产科杂志》1997年第7期412-414,共3页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的：对１９８５～１９９５年在我院尸体检查证实为畸形死亡的３９例围产儿石蜡包埋组织标本，进行有关病原体的检测，以明确围产儿致畸原因。方法：用改良的水浴脱蜡、直接裂解及酶解法，进行石蜡包埋的死亡围产儿肝、脑组织的弓形体、巨细胞病毒及Ⅱ型单纯疱疹病毒的聚合酶链反应（ＰＣＲ）检测。同时对送检胎盘者，取胎盘石蜡包埋组织，分别作上述病原体的检测。结果：３９例中２５例检出病原体，检出率为６４％。其中泌尿生殖系统畸形１３例中９例检出病原体，神经管缺陷１２例中８例检出病原体。此两种畸形中病原体的检出率高达６８％。结论：本组资料证实宫内感染是围产儿致畸的重要原因之一，并对胎盘感染常见的形态学变化及畸形儿胎盘常规送检的重要性进行了讨论。Objective: To examine 3 types of pathogens in dead fetus with congenital defects for exploring the pathogenesis. Methods: The paraffin embeded brain and liver tissues from 39 fetal autopsies with congenital defects were examined for toxoplasma, cytomegalovirus, and herpes simplex Ⅱ by polymerase chain reaction. Among them 16 placentae were examined as well. Results: Pathogens were detected in 25 of 39 cases, a positive rate of 64%. Nine out of the 13 fetuses with urogenital defects were positive for the above pathogens, while 8 from the 12 with neural tube defects were positive as well. Conclusion: Intrauterine infection was one of the important causes of birth defects. The characteristics of placenta morphologic changes were described, and the significance of placenta investigation in congenital malformation was emphasized.

关 键 词：畸胎 PCR 病理学 DNA 石蜡包埋组织 

分 类 号：R714.53[医药卫生—妇产科学] R446.9[医药卫生—临床医学]