促超排卵周期胰岛素样生长因子-1对人卵泡发育的调控  

Insulin Growth Factor I in the Development of Ovarian Follicles

作　　者：魏志新[1,2] 张丽珠[1,2] 李美芝[1,2] 杨池荪[1,2] 贺师鹏[1,2] 张小为[1,2] 

机构地区：[1]北京医科大学第三医院妇产科 [2]北京医科大学生物物理教研室

出　　处：《中华妇产科杂志》1997年第8期486-488,共3页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的：探讨促超排卵周期胰岛素样生长因子－１（ＩＧＦ－１）对人卵泡发育的调控。方法：采用放射免疫法检测体外受精－胚胎移植（ＩＶＦ－ＥＴ）患者体液（１３例用药前血清、３８例卵泡液及同期血清）中ＩＧＦ－１及性激素的含量；采用原位杂交技术检测５例在ＩＶＦ周期得到的颗粒细胞上ＩＧＦ－１及其受体（ＩＧＦ－１Ｒ）的表达。结果：（１）促超排卵过程中血清ＩＧＦ－１含量显著增高（Ｐ＜０．００１），其在卵泡液内的平均含量低于同期血清中水平（Ｐ＜０．０５），且与卵泡发育数之间呈负相关关系（Ｐ＜０．０１）；在卵泡发育数少于３个的卵巢低反应型患者中，卵泡液水平接近同期血清水平。（２）卵泡液内ＩＧＦ－１与卵泡刺激素（ＦＳＨ）、同期末梢血清中ＩＧＦ－１与雌二醇（Ｅ２）呈显著的负相关（Ｐ＜０．０１）。（３）颗粒细胞上存在ＩＧＦ－１Ｒ，但不能产生ＩＧＦ－１。结论：促超排卵药物作用后血清ＩＧＦ－１水平升高，并进入卵泡液内协同促性腺激素参与对卵泡发育的调控。Objective: To investigate the role of insulin growth fator 1 (IGF 1) in the development of ovarian follicles under the stimulation of gonadotropins in an in vitro fertilization (IVF) program. Methods: Radioimmuoassay was used to determine the levels of sex hormones and IGF 1 in the serum and follicular fluid samples. In situ hybridyzation was used to detect the expression of IGF 1 and IGF 1 receptor (IGF 1R) in the granulosa cell obtained from follicular aspiration in women undergoing IVF egg retrieval procedures. Results: (1) Levels of plasma IGF 1 increased with the development of follicles ( P <0.001); follicular fluid (FF) IGF 1 levels were lower than those of matched plasma; FF IGF 1 decreased with increae of follicle numbers ( P <0.01), e.g. in patients with less than 2 follicles (diameter ≥15 mm) at the tine of ovum pick up the FF IGF 1 levels were much higher than patients with more than 2 follicles ( P <0.05). (2) Significant negative correlation was found between plasma IGF 1 and plasma E 2 and also between FF IGF 1 and FF follicle stimulating hormone (FSH) druing ovum pick up ( P <0.01). (3) IGF 1 mRNA was not, while IGF 1R mRNA was found to be present in the human granulosa cells taken at ovum pick up. Conclusions: Plasma IGF 1 production might be stimulated by gonadotropins and distributed into the ovarian follicles by diffusion from peripheral circulation, acting upon the granulosa cells and therefore plays a complementary role with the gonadotropins in the regulation of follicular development.

关 键 词：超排卵 卵泡液 卵泡发育 性激素 IGF-1 

分 类 号：R714.13[医药卫生—妇产科学]