肿瘤病人血清中癌细胞凝集因子单克隆抗体的制备  

PREPARATION OF McAb AGAINST CANCER CELL AGGLUTINATING FACTOR(CCAF)

作　　者：侯琳[1,2,3] 任晓燕[1,2,3] 周柔丽 

机构地区：[1]青岛医学院生物化学教研室 [2]青岛医学院病理生理学教研室 [3]北京医科大学细胞生物学教研室

出　　处：《青岛医学院学报》1997年第3期216-217,共2页Acta Academiae Medicinae Qingdao Universitatis

基　　金：卫生部科研基金

摘　　要：①目的通过制备癌细胞凝集因子的单克隆抗体（ＭｃＡｂ），进一步研究该凝集因子的结构、功能、组织来源，并为临床建立检测试剂盒打下基础。②方法采用杂交瘤技术将免疫小鼠脾细胞与ＳＰ２／Ｏ细胞融合，经ＥＬＩＳＡ法结合癌细胞凝集抑制实验筛选出阳性克隆。③结果融合阳性率７８．１％；筛选出的杂交瘤细胞染色体数目为９６～１０６条，ＳＰ２／０骨髓瘤细胞为６８条，小鼠脾细胞为４０条，得到的ＭｃＡｂ亚类分别是ＩｇＧ１（Ｅ９）和ＩｇＧ２α（Ｅ１１）．④结论获得了２株既能分泌抗体，又能阻断癌细胞凝集的杂交瘤细胞株（Ｅ９和Ｅ１１）。Objective To prepare a hybridoma cell line which secret McAb against CCAF and can be used for further study of CCAFs chemical structure, function and tissue origin in order to provide theoretical data for preparation of laboratory kit. Methods Spleen cells from BALB/C mice immunized with CCAF were fused with SP 2/O myeloma cells. Positive clones were screened by ELISA combined with the test of inhibiting cancer cell aggregation. Results The fusion positive rate was 78.1%. The number of hybridoma cell chromosome was from 96￣106, The McAb subclasses were IgG 1(E 9) and IgG 2α (E 11 ), respectively. Conclusion The present experiment successfully obtained 2 strains of hybridoma cells which could either secrete McAb against CCAF or inhibit cancer cell agglutination.

关 键 词：抗体 单克隆 肿瘤转移 细胞凝集因子 

分 类 号：R392－33[医药卫生—免疫学] R73－37[医药卫生—基础医学]