河南省不同产地3种菊头蝠RAPD分析  

RAPD analysis of 3 Rhinolophus bats from different localities in Henan

作　　者：余燕[1] 马金友[1] 牛红星[2] 王艳梅[2] 

机构地区：[1]河南科技学院动物科学学院,河南新乡453003 [2]河南师范大学生命科学学院,河南新乡453007

出　　处：《广西农业生物科学》2008年第2期99-103,共5页Journal of Guangxi Agricultural and Biological Science

基　　金：河南省科技攻关项目(0224070051)

摘　　要：用随机引物对河南省角菊头蝠(Rhinolophus cornutus)、不同产地的中菊头蝠(Rhinolophus affinis)和马铁菊头蝠(Rhinolophus ferrumequinum)进行DNA多态性研究,以探讨它们之间的亲缘关系。从20个随机引物中优化出15个引物对基因组DNA进行扩增,10个标本共扩增出184条DNA谱带,平均每个引物扩增出12条谱带,其中多态性谱带173条,多态率为94%。聚类结果表明:对同种蝙蝠,同一地理区域的个体之间分化较小,不同地理区域的个体之间分化较大;中菊头蝠与角菊头蝠之间的亲缘关系较近,而与马铁菊头蝠之间的亲缘关系较远。Random Amplification Polymorphic DNA （RAPD） was used to study the phylogenetic relationship of Rhinolophus cornutus, Rhinolophus a f finis and Rhinolophus ferrumequinum from different localities in Henan Province. Fifteen primers were screened from 20 random primers and 184 DNA fragments were amplified from 10 specimen, 173 （94%） of which were polymorphic. The phylogenetic tree indicated that： there was a little differentiation among bats in the same area, while there was significant differentiation between the different areas; Rh. a f finis had nearer relationship with R. cornutus and further relationship with Rh. ferrumequinum.

关 键 词：随机扩增多态性DNA(RAPD) 菊头蝠属 亲缘关系 河南 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S185[农业科学—植物保护]