“海花1号”花生核型的研究  

Study on the Karyotype of Peanut Variety Haihua 1

作　　者：庞书香[1] 张秀芳[2] 庞秋香[2] 

机构地区：[1]济宁职业技术学院生化系,山东济宁272037 [2]山东理工大学生命科学院,山东淄博255049

出　　处：《安徽农业科学》2008年第15期6256-6257,6264,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金资助项目(30700071)

摘　　要：[目的]分析“海花1号”花生核型,积累花生种质资源的细胞学资料。[方法]以“海花1号”为试材,当根尖长到1-2 cm时,进行预处理后固定,酶解抽取细胞悬液制作载玻片,选取分散良好、显示清晰的染色体组核型,拍照后用Adobe Photoshop7.0进行核型分析。[结果]“海花1号”染色体数目为2n=40,有1对随体染色体且随体在第15对染色体上。“海花1号”花生的染色体核型公式为：K（2n）=40=36m＋2m（SAT）＋2Sm。“海花1号”花生的染色体相对长度组成为2n=40=40L。相对长度变异范围为2.22-3.57,差值为1.35。全组染色体总长为96.16μm,“海花1号”花生的As.K%=60.44%。[结论]“海花1号”花生为二倍体植物（2n=40）,染色体都是长染色体,有一定程度的不对称性。[Objective] The study aimed to analyze karyotype of peanut variety Haihua 1 and accumulate the cytological data of peanut germplasm resources.[Method] With Haihua 1 as tested material,after its root tip,1～2 cm length,was pretreated,fixed and hydrolyzed with enzyme,cell suspension was extracted to make the object plate.Then object plates with good dispersion and clear karyotype were collected and taken photo for karyotype analysis by Adobe Photoshop7.0.[Result] Haihua 1 had the chromosome numbers of 2n=40 and had a pair of satellite chromosome in 15th pair of chromosome.Karyotype formula of Haihua 1 was K（2n）=40=36 m＋2 m（SAT）＋2Sm.The relative length of chromosome relative length of Haihua 1 ranged from 2.22 to 3.57 and its difference value was 1.35.The total length of chromosome was 96.16 μm and As.K % of Haihua 1 was 60.44%.[Conclusion] Haihua 1 was diploid（2n=40）,and its chromosomes were all long,with dissymmetry to some degree.

关 键 词：“海花1号” 花生 核型 

分 类 号：S565.2[农业科学—作物学]