活性氧不敏感型拟南芥突变体的遗传特性分析  

Analysis on Genetic Characters of Arabidopsis thaliana Mutant Insensitive to Reactive Oxygen

作　　者：何金环[1] 李巧枝[1] 

机构地区：[1]郑州牧业工程高等专科学校生物工程系,河南郑州450011

出　　处：《安徽农业科学》2008年第15期6258-6258,6312,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]研究活性氧不敏感型拟南芥突变体的遗传特性。[方法]拟南芥种子经消毒后,在MS固体培养基上进行培养。培养5-6d后,将幼苗移栽于蛭石上继续培养。开花时,以野生型为父本,突变体为母本,进行杂交,成熟时收获得到F1代种子。将F1种子种于MS培养基上,生长5-6 d后,用10^-4mol/L的H2O2处理幼苗,用根弯曲法检测植株对H2O2抗性表现,进行遗传分析。[结果]F1代幼苗,全部不抗H2O2,即根的生长受到抑制。在243个F2代植株中,有184株不抗H2O2,59株抗H2O2,不抗与抗的植株数呈现3∶1的分离。[结论]活性氧不敏感型拟南芥突变体发生了隐性单基因突变。[Objective] The study aimed to explore the genetic characters of Arabidopsis thaliana mutant insensitive to reactive oxygen.[Method] The seeds of A.thaliana after being sterilized were cultivated in the MS solid culture medium.After cultivated for 5～6 d,the seedlings were transplanted in vermiculite and cultivated again.The hybridization was made with the wild type as male and the mutant as female in flowering stage.The seeds of F1 were planted in MS culture medium.After growing for 5～6 d the seedlings were treated with 10-4 mol/L H2O2,the resistance of seedlings to H2O2 was detected by the root bending method and the genetic characters of the mutants were analyzed.[Result] The total seedlings of F1 were nonresistant to H2O2,i.e.the growth of roots was restrained.184 plants were nonresistant to H2O2,while 59 plants were resistive to H2O2 among 243 plant in F2.The separation ratio of nonresistant plant to resistant plants was 3∶1.[Conclusion] There was a single recessive gene mutation happened in A.thaliana mutant insensitive to reactive oxygen.

关 键 词：活性氧 拟南芥 突变体 

分 类 号：S312[农业科学—作物栽培与耕作技术]