海藻糖对低温储存皮肤β1 integrin及活力的影响  

Investigation on the effects of trehalose on β1 integrin and viability of human skin

作　　者：贾晓明[1] 马彩虹[1] 于瑜[1] 李东杰[1] 姚咏明[1] 董宁[1] 

机构地区：[1]解放军总医院304临床部全军烧伤研究所,北京100037

出　　处：《军医进修学院学报》2008年第3期206-208,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金项目(30070298;30672177);首都医学发展科研基金项目(2005-3087)

摘　　要：目的:比较海藻糖与传统低温保护剂对皮肤组织β1整合素(β1 integrin)低温保存后表达的影响,揭示海藻糖的低温抗冻机制。方法:新鲜成人皮肤组织分为2组,海藻糖-二甲基亚砜(T-D)、二甲基亚砜-丙二醇(D-P)作为低温保护剂保存,-196℃液氮冻存7 d、14 d复温,设新鲜组为对照组,免疫组织化学染色对各组间皮肤进行比较。RT-PCR方法检查β1 integrin基因表达水平。采用皮肤内琥珀酸脱氢酶的定量测定法(SDH)检测两种低温保护剂组合对皮肤活力影响,并通过透射电镜观察不同低温保护剂对皮肤基底膜变化的影响。结果:结果显示T-D能够很好保护皮肤组织,T-D 7 d组β1 integrin的基因表达量(0.769±0.052)与新鲜皮肤组(0.789±0.082)相似。琥珀酸脱氢酶(18.360±3.060)与新鲜组(20.517±1.976)无明显差异。结论:T-D对基底膜有优势保护作用,海藻糖对β1 integrin的保护作用在海藻糖的低温抗冻机制中发挥重要作用。Objective： To study the effects of different cryoprotectants on human skin,in order to offer experiment gist for selecting the best cryoprotectants. Methods：The skins to be cryopreserved were first treated with DMSO-propyleneglycol, trehalose-DMSO, respectively, to be compared with fresh skin. Then the histological structure of skin of different groups was observed and analyzed by pathological technology （immunohistochemical staining）. Furthermore, we investigated the influence of trehalose on β1 integrin at gene level with RT-PCR. The viability of skin in five respective groups was evaluated using SDH assay. The experiments were carried out 7 days and 14 days after cryopreservation. Results： The results of histological studies showed that β1 integrin could be well-protected by trehalose. The results of RT-PCR test and SDH assay also revealed that skin cryopreserved with trehalose-DMSO was as good as fresh skin, and better than other groups. Conclusion：The results suggested that cryopreservation protocol-trehalose-DMSO is better than the traditional cryoprotectant for cryopreservation on β1 integrin of human skin.

关 键 词：海藻糖 皮肤 Β1整合素 低温保护剂 

分 类 号：R329.499[医药卫生—人体解剖和组织胚胎学]