斑点叉尾鮰烂鳃病病原柱状黄杆菌的分离及鉴定  

Isolation and Classification of Pathogenic Bacterium Caused by Gill-rote Disease in Channel Catfish(Ictalunes punctatus)

作　　者：刘礼辉[1] 李宁求[1] 石存斌[1] 潘厚军[1] 付小哲[1] 吴淑勤[1] 

机构地区：[1]中国水产科学研究院珠江水产研究所

出　　处：《安徽农业科学》2008年第17期7124-7126,共3页Journal of Anhui Agricultural Sciences

基　　金：国家支撑计划项目(2006BAD03B05);广东省自然科学基金项目(06024033)

摘　　要：[目的]对从斑点叉尾鮰烂鳃病中分离的病原菌进行分类学地位鉴定。[方法]采用人工感染确定其病原性,常规生理生化鉴定和16SrRNA基因序列分析方法进行分析。[结果]该菌株为革兰氏阴性杆菌,滑行运动,无鞭毛,菌体大小为(0.3～0.5)μm×(5～10)μm,过氧化氢酶反应阳性,不能发酵和利用葡萄糖等多糖,不水解酪氨酸,不产生吲哚。所测16SrRNA基因序列经BLAST分析表明,与GeneBank上登录的柱状黄杆菌的16SrRNA序列同源性最高,其相似性达98.2%。基于16SrRNA基因序列构建的系统进化树表明,菌株GHS061212与柱状黄杆菌(AY841899)聚为1个分支,且置信度较高,为71.0%。[结论]菌株GHS061212被鉴定为柱状黄杆菌,这是国内首次报道斑点叉尾鮰烂鳃病病原为柱状黄杆菌。[Objective] The research aimed to classify the pathogenic bacterium （GHS061212） isolated from the gill-roll disease in lctalunes punctatus. [Method] Its pathogenicity was confirmed by artificial infection, and it was analyzed by using physiological and biochemical identification and 16S rRNA＇gene sequence analysis.[Result] The strain was non-flagellated gram-negative rod, gliding motility, 0.3-0.5μm in width and 5-10μm in length, catalase positive. No acid produced from carbohydrates, tyrosine were not hydrolyzed, no indole produced. The analysis on 16S rRNA gene sequence obtained showed the highest level of similarity to 16S rRNA gene sequence of Flavobacterim columnare in blast, and their nucleotide identities were about 98.2%. Phylogenetic tree of Flavobacterim columnare based on 16S rRNA gene indicated that the strain GHS061212 clustered together with Flavobacterium columnare （AY841899 ）, had high bootstrap which was 71.0%.[Conclusion] The strain GHS061212 was identified as Flavobacterium columnare. It was the first report that this bacterium caused by the gill-roll disease in lctalunes punctatus in China.

关 键 词：斑点叉尾鲴 烂鳃病 柱状黄杆菌 生理生化鉴定 16S RRNA基因序列分析 

分 类 号：S512.1[农业科学—作物学] S965.199