福建柏挥发油的化学成分及其生物活性研究  

Study on the Chemical Constituents and Biological Activities of Volatile Oil from Fokienia hodginsii

作　　者：张艳平[1] 杨守晖[1] 曹奇龙[1] 张文慧[1] 陈凤美[1] 

机构地区：[1]徐州师范大学江苏省药用植物生物技术重点实验室,江苏徐州221116

出　　处：《安徽农业科学》2008年第17期7290-7291,7298,共3页Journal of Anhui Agricultural Sciences

基　　金：国家"十一五"支撑项目"城市人居生态林构建关键技术研究"(2006BAD03A0603);徐州侧柏生态环境景观林配置功能及效益研究(XZ2006221)

摘　　要：[目的]测定福建柏挥发油的化学成分及其生物活性,了解福建柏是否适用于城市生态保健型绿化模式的栽培树种。[方法]采用水蒸气蒸馏法提取挥发油;采用气相色谱-质谱(GC-MS)联用技术分析福建柏叶的挥发油成分,应用色谱峰面积归一法分析各成分的质量分数;采用杯碟法和Alamar Blue法进行生物活性测定。[结果]从挥发油检出14个峰,被确认为14种化合物,含量较高的物质有:β-橙椒烯(22.514%)、τ-杜松醇(19.883%)、石竹烯(10.133%)等;体外生物活性实验表明,该挥发油对大肠杆菌无抑制作用,对金黄色葡萄球菌和红酵母有一定的抑制能力,AlamarBlue法测定福建柏挥发物质对人非小细胞肺癌(NCI-H460)细胞的抑制作用不大,浓度为100μg/ml时抑制率为(24.01±8.01)%。[结论]福建柏适用于城市生态保健型绿化模式栽培的树种。[Objective] The chemical constituents and biological activity of volatile oil were tested in order to find out the ecological function of Fokienia hodginsii. [Method] The volatile oil was extracted from the leaves of Fokienia hodginsii by steam distillation and its chemical constituents were analyzed by GC-MS method; the biological activity was tested by the method of Oxford Cup and Alamar Blue. [Rusuh] 14 constituents were detected, and 14 compounds of the volatile oil were identified, the main compounds including β-cubebene （22.514%）, τ-cadinol （19.883%） and caryophyllene （10.133%）. The antibacterial experiments of the oil showed that the volatile oil had antimicrobial activities to Staphylococcus aureus and Rhodotorula glutinis, but had no inhibition against Escherichia coil, the result of antitumor activities in vitro against NCI-H460 by Alamar Blue method indicated that the inhibitory rate was （24.01 ±8.01）% at the volatile oil concentration of 100 μg/ml. [Conclusion] Fokienia hodginsii was suitable for ecological healthy virescence mode in cities.

关 键 词：福建柏 挥发油 化学成分 生物活性 

分 类 号：S791.43[农业科学—林木遗传育种]