不同发育阶段甜樱桃花授粉后花粉萌发和花粉管生长的差异  

Discrepancy of pollen germination and pollen tube growth at different mature degree of pistil in sweet cherry

作　　者：李晓[1] 吴华清[1] 吴俊[1] 陶书田[1] 张绍铃[1] 

机构地区：[1]南京农业大学园艺学院,南京210095

出　　处：《果树学报》2008年第4期501-505,共5页Journal of Fruit Science

基　　金：高校博士学科点专项科研基金(NO.B200523)

摘　　要：选用自交不亲和性程度不同的2个甜樱桃品种莫利和拉宾斯为试材,分别在开花前、开花当天和开花后进行莫利自花、拉宾斯自花和莫利×拉宾斯异花授粉,并于授粉后12、24、48、72、96h切取花柱,用FAA固定,荧光染色后压片观察。结果表明,3个组合的花粉萌发率都以开花当天授粉的最佳,拉宾斯自交和莫利×拉宾斯的花粉管生长状况也以开花当天授粉的最佳,但莫利自交的花粉管生长状况却以花前第4天最佳,花后第4天次之,而当天授粉的最差。因此认为,甜樱桃自交不亲和程度与其雌蕊的发育状态密切相关,蕾期授粉和延迟授粉都能够在一定程度上克服甜樱桃的自交不亲和性,但蕾期授粉的作用更加明显。Pollen germination and pollen tube growth in the styles of two sweet cherry cultivars, Moreau and Lapins, were examined after self- and cross- pollination among different florescence respectively. At intervals of 12, 24, 48, 72 and 96 h after pollination, the pollen germination and pollen tubes subsequent growth in the styles was observed with fluorescence microscope after fixed in FAA and coloration. Results showed that the best pollen germination percentage of the self-pollinated Moreau and Lapins, and cross-pollinated Moreau × Lapins all occurred on the day of anthesis. The best pollen tube growth was also of those pollinated on the anthesis. But the pollen tube growth of self-pollinated Moreau was in contrary. Its best pollen tube growth was that pollinated 4 d before flowering, then that of pollinated 4 d after flowering, while that pollinated on anthesis was the worst. So it is believed that the self-incompatibility of sweet cherry was correlated with the development of the pistils. Pollination carried out at pre-flowering stage and delayed-pollination could overcome the self-incompatibility to some extent, but pollination at pre-flowering day should be better.

关 键 词：甜樱桃 自交不亲和 蕾期授粉 延迟授粉 

分 类 号：S662.5[农业科学—果树学]