中国小麦育成品种和农家种中慢锈基因Lr34/Yr18的分子检测  

Molecular Characterization of Slow-Rusting Genes Lr34/Yr18 in Chinese Wheat Cultivars

作　　者：杨文雄[1] 杨芳萍[1] 梁丹[2] 何中虎[2] 尚勋武[3] 夏先春[2] 

机构地区：[1]甘肃省农业科学院作物研究所,甘肃兰州730070 [2]中国农业科学院作物科学研究所/国家小麦改良中心 [3]甘肃农业大学农学院,甘肃兰州730070

出　　处：《作物学报》2008年第7期1109-1113,共5页Acta Agronomica Sinica

基　　金：西部之光人才培养计划;国家科技支撑计划项目(2006BAD01A02-29);国家高技术研究发展计划(863计划)项目(2006AA10Z1A7,2006AA100102);甘肃省生物技术研究及应用项目(GNSW-2007-19)

摘　　要：Lr34/Yr18是重要的慢叶锈和慢条锈基因,携带该连锁基因的小麦品种被广泛种植于世界许多国家。利用STS标记csLV34对慢叶锈和慢条锈基因Lr34/Yr18进行分子检测的结果表明,我国231份育成品种(系)中仅有14份材料携带Lr34/Yr18基因,占6.1%。不同麦区分布频率不同,其中北部冬麦区为零,黄淮冬麦区、长江中下游冬麦区、西南冬麦区和西北春麦区分别为3.0%、21.4%、16.7%和33.3%。在422份农家种中,359份含有Lr34/Yr18基因,占85.1%。Lr34/Yr18基因在不同麦区的分布频率也存在差异,北部冬麦区、黄淮冬麦区、长江中下游冬麦区、西南冬麦区、南部冬麦区和西北春麦区分别为89.6%、77.4%、93.1%、93.8%、96.6%和61.1%。csLV34标记扩增产物为150bp和229bp的片段,能有效鉴别品种是否携带Lr34/Yr18基因,是一个重复性好、准确率高的分子标记,可用于小麦Lr34/Yr18基因的鉴定与选择。Lr34 and Yr18 are important slow rusting resistance genes that have been widely used in many countries. The aim of this study was to identify Lr34/Yr18 genes in 231 Chinese wheat cultivars and 422 landraces using an STS marker csLV34, which can amplify a 150-bp and a 229-bp fragment in the genotypes with and without Lr34/Yr18, respectively. The results indicated that only 14 genotypes carried the resistance genes Lr34/Yr18 in the improved wheat cultivars, with a frequency of 6.1%. Different frequencies were found in wheat cultivars from different zones, which were 0, 3.0%, 21.4%, 16.7%, and 33.3% in North Plain Winter Wheat Region （NPWWR）, Yellow-Huai Facultative Winter Wheat Region （YHFWWR）, Middle-Lower Yangtze Valley Winter Wheat Region （MLYVWWR）, Southwest Winter Wheat Region （SWWR）, and Northwest Spring Wheat Region （NSWR）, respectively. Whereas, 85.1% of 422 landraces contained Lr34/Yr18, with the frequencies of 89.6%, 77.4%, 93.1%, 93.8%, 96.6%, and 61.1% in NPWWR, YHFWWR, MLYVWWR, SWWR, Southern China Winter Wheat Region （SCWWR） and NSWR, respectively. The marker csLV34 exhibited a good repeatability, and can be therefore used for the identification of Lr34/Yr18 in wheat breeding programs.

关 键 词：普通小麦 Lr34/Yr18基因 STS标记csLV34 分子标记辅助选择 

分 类 号：S512.1[农业科学—作物学] Q343[生物学—遗传学]