低磷胁迫下水稻产量性状变化及其QTL定位  

Yield Trait Variation and QTL Mapping in a DH Population of Rice under Phosphorus Deficiency

作　　者：穆平[1] 黄超[1] 李君霞[1] 刘立峰[1] 刘弋菊[1] 李自超[1] 

机构地区：[1]中国农业大学农业部作物基因组学与遗传改良重点实验室/北京市作物遗传改良实验室

出　　处：《作物学报》2008年第7期1137-1142,共6页Acta Agronomica Sinica

基　　金：国家高技术研究发展计划(863计划)项目(2006AA10Z158,2006AA100101);国家科技支撑计划项目(2006BAD13B01);引进国际先进农业科学技术计划(948计划)项目[2006-G1(B)];山东省中青年科学家科研奖励基金项目(620632)

摘　　要：为研究低磷胁迫对水稻产量及其构成因素QTL表达的影响,以耐低磷旱稻IRAT109和磷敏感水稻越富杂交的116个株系的DH群体为材料,在低磷和正常栽培条件下,调查了千粒重、结实率、有效穗数、穗粒数及单株产量等性状。结果表明,结实率、有效穗数和单株产量对低磷胁迫的敏感性较大,而千粒重、穗粒数对低磷胁迫的敏感性较小。利用水稻分子连锁图中94个RFLP标记和71个SSR标记,依据以上5性状低磷胁迫下与对照的差值进行QTL定位。共检测出17个QTL,其中12个对表型变异的贡献率大于10%。3、6和7号染色体上3个标记区域存在QTL成簇分布,这些高贡献率QTL及成簇分布QTL可作为水稻耐低磷产量性状分子育种的重要候选区域。To study the difference of QTLs for rice yield and its component traits under different phosphorus applications, a DH （doubled haploid） population including 116 lines, derived from a cross between phosphorus deficiency tolerant rice IRAT109 and phosphorus deficiency sensitive rice Yuefu, was evaluated for yield per plant （YP） and its components including 1000-grain weight （GW）, seed-setting percentage （SP）, panicle number per plant （PN）, and grain number per panicle （GN） under phosphorus deficiency and normal conditions. No significant differences were found in GW and GN but significant differences were found in SP, PN, and YP for the DH population between the two conditions. This result indicted that SP, PN, and YP were more easily influenced by phosphorus deficiency than GW and GN. A total of seventeen QTLs were detected for plant yield and its components under phosphorus deficiency using the constructed molecular linkage map （including 94 RFLP and 71 SSR markers and covering 1 535 cM）. Among them, there were twelve QTLs with general contributions of over 10% to phenotypic variation. Three tightly linked QTL regions on chromosomes 3, 6, and 7 were identified. These QTLs with high general contribution and tightly linked QTL regions might be useful for phosphorus deficiency tolerant and high yielding rice breeding by molecular marker assisted selection.

关 键 词：水稻 低磷胁迫 产量性状 QTL 

分 类 号：S511[农业科学—作物学]