典型抗生素废水净化菌株的分离筛选及其效果研究  

Isolation and screening of bacterial strains during treatment of a typical antibiotic wastewater and study of their effect

作　　者：王立群[1] 孙文[1] 章广德 曹立群[1] 顾文杰[1] 肖维伟[1] 喻其林[1] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030 [2]哈尔滨市环境工程评估中心,哈尔滨150076

出　　处：《中国农业大学学报》2008年第4期97-101,共5页Journal of China Agricultural University

基　　金：黑龙江省科技公关项目(GC05C208)

摘　　要：为使β-内酰胺环类抗生素生产废水得以有效处理,本研究以该类废水制作分离培养基,采用摇瓶实验模拟好氧曝气阶段,从活性污泥分离、筛选效应菌株,并对其进行鉴定,确定最佳作用条件,验证对该废水有机物的降解效果。实验结果显示:分离、筛选到B4、B5、B2和B7共4株对该废水有机物具有高效降解作用并对β-内酰胺环类抗生素有耐受能力的细菌,分别归类于不动杆菌属(Acinetobacter)、假单胞菌属(Pseudomonas)、埃希氏菌属(Escherichia)和芽孢杆菌属(Bacillus);通过正交试验得出,温度是影响有机物降解效果的首要因素,其次是摇床转速;在摇瓶模拟的最佳作用条件下:即温度35℃、转速150 r/min和初始pH 7.0时,加入效应菌株组合的试验组有机物降解力比对照组提高10%左右,并使整个体系对有机物变化冲击的抗力增强。In order to raise the biodegradation value ofβ- lactam antibiotic wastewater, an aerobic phase was simulated with a shaking flask. The effect of strains isolated from the activated sludge of wastewater treatment plant was studied to define the best condition using an orthogonal experiment. The degradation after adding the strains was validated. Four bacteria were isolated that can degrade organics and resist the β-lactam antibiotic. The strains were Escherichia, Acinetobacter,Pseudomonas,Bacillus. The optimal operational conditions were as follows： cultivation temperature, 35 ℃ , shaking speed, 150 r/min and initial pH, 7.0. The percentage removal of organics was about 10% higher than the control group and performed well in different concentrations of wastewater.

关 键 词：β-内酰胺环类抗生素 废水处理 CODGr值 分离与筛选 效应菌株 

分 类 号：X703[环境科学与工程—环境工程]