出生前氚水照射对仔鼠海马神经元电生理及神经行为的影响  

EFFECTS OF PRENATAL LOW DOSE BETA RADIATION FROM TRITIATED WATER ON RAT HIPPOCAMPUS NEURONS: ELECTROPHYSIOLOGICAL AND NEUROBEHAVIOURAL CHANGES

作　　者：高卫民[1] 周湘艳 何湘平[2] 刘传缋[2] 

机构地区：[1]卫生部工业卫生实验所 [2]军事医学科学院毒物药物研究所

出　　处：《辐射防护》1997年第5期363-372,共10页Radiation Protection

摘　　要：妊娠１３ｄ的大鼠接受累积吸收剂量分别为０．０００、０．０４４、０．０８８和０．２６４Ｇｙ的氚水照射，观察对新生大鼠体外培养海马神经元形态、细胞计数、最大Ｃａ２＋电流的影响，并通过Ｙ迷宫刺激回避反射与条件反射试验的学习记忆行为进行综合评价。结果表明，出生前接受０．０８８Ｇｙ的氚水照射即可减少体外培养海马神经元的数目；随剂量的增加，Ｃａ２＋电流有下降趋势，剂量达０．２６４Ｇｙ时导致Ｃａ２＋电流显著降低；Ｙ迷宫刺激回避反射与条件反射试验结果表明，剂量达０．０８８Ｇｙ时即可导致仔鼠学习记忆行为受损。Pregnent Wistar rats were exposed to tritiated water (HTO) on day 13 of gestation so that for their offsprings,the absorbed doses were estimated to be 0.000 ,0.044,0.088 and 0.264 Gy. The influence of HTO to the morphology and number of hippocampus pyramidal neurons and the maximum electric current of Ca 2+ in neurons was observed for the in vitro cultured hippocampus of new born rats and the learning and memory behaviours were assessed by the electric avoidance reflex test in a Y maze and the condition reflex test for young rats. The results show that prenatal exposure to HTO in a cumulative dose of 0.088 Gy can cause a reduction in number of neurons in hippocampus cultured in vitro, and that the electric current of Ca 2+ tends to decline with cumulative dose increasing,with the significant decrease in offsprings prenatally exposed to HTO in dose of 0.264 Gy . The results of electric avoidance reflex test in a Y maze and condition reflex test indicate that for young rats prenatally exposed to HTO,a cumulative dose of 0.088 Gy could induce damage in their learning and memory behaviours. (

关 键 词：海马神经元 神经行为 学习记忆行为 氚水照射 

分 类 号：R818.03[医药卫生—放射医学] TL75[医药卫生—临床医学]