花鲈冷冻精液受精鱼苗与鲜精受精鱼苗的同工酶比较分析  

Isozyme Analysis of Spotted Sea bass (Lateolabrax maculatus) Artificially Fertilized by Cryopreserved Spermatozoa and Normal Spermatozoa

作　　者：陈艳翠[1] 高天翔[1] 陈松林[2] 季相山[2] 田永胜[2] 邓寒[2] 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003 [2]中国水产科学研究院黄海水产研究所农业部海洋渔业资源可持续利用重点实验室,山东青岛266071

出　　处：《淡水渔业》2008年第3期27-31,共5页Freshwater Fisheries

基　　金：国家863项目(2001AA621100);国家自然科学基金项目(39970578);山东省博士基金项目(02BS018)资助

摘　　要：采用水平凝胶电泳技术对花鲈(Lateolabrax maculatus)冷冻精液受精鱼苗与鲜精受精鱼苗进行了同工酶分析。本实验选取肌肉和肝脏两种组织,检测了IDHP、PGM、LDH、GPI、MDH、G3PDH、ME、HK、SOD、G6PD和SDH等11种酶,得到13个位点:IDHP*、PGM-1*、PGM-2*、LDH*、GPI-1*、GPI-2*、MDH*、G3PDH*、ME*、HK*、SOD*、G6PD*和SDH*。结果显示在0.99水平,SDH*和PGM-2*位点在两种受精鱼苗中均表现为多态,多态位点比例均为0.1538;平均预期杂合度分别为0.0460和0.0407,但未观测到杂合子,观测杂合度均为0,遗传偏离指数均为-1;有效等位基因数分别为1.0720和1.0850,花鲈冷冻精液受精鱼苗与鲜精受精鱼苗的遗传相似度为0.9991,遗传距离为0.0010。由此可见,花鲈的冷冻精液受精鱼苗和鲜精受精鱼苗间未出现同工酶水平上的遗传差异,冷冻保存精液完全适用于花鲈育苗生产。Horizontal starch gel electrophoresis was used to investigate the genetic difference on isozyme level between spotted sea bass artificially fertilized by cryopreserved spermatozoa and normal spermatozoa. Muscle and liver samples from two populations were chosen for the isozyme electrophoresis. 13 putative enzymes coding loci（ IDHP＊ , PGM-1 ＊ , PGM- 2＊, LDH＊, GPI-1＊, GPI-2＂, MDH＊, G3PDH＊, ME＊, HK＊, SOD＊, G6PD＊andSDH＊ ）were obtained based on 11 enzymes（IDHP, PGM, LDH, GPI, MDH, G3PDH, ME, HK, SOD, G6PD and SDH）examined in this experi- ment and two of them were determined to be polymorphic, i.e. , SDH＊ and PGM-2＊ when the most common allele is less than or equal to 0. 99. The proportions of polymorphic loci of spotted sea bass artificially fertilized by cryopreserved spermatozoa and normal spermatozoa were both 0. 1538, respectively. There was no heterozygote appeared in individuals, and the average observed heterozygosities was therefore 0. The expected heterozygosities were 0. 0460 and 0. 0407, respectively for spotted sea bass artificially fertilized by cryopreserved spermatozoa and normal spermatozoa. The Nei＇ s genetic dis- tance of two populations was 0. 0010. The average effective numbers of alleles were 1. 0720 and 1. 0850, respectively.The results showed that there was no difference on isozyme level in the spotted sea bass artificially fertilized by cryopreserved spermatozoa and normal spermatozoa, so the cryopreserved spermatozoa is fit for spotted sea bass production completely.

关 键 词：花鲈(Lateolabrax maculatus) 冷冻精液受精鱼苗 鲜精受精鱼苗 同工酶 

分 类 号：Q959.473[生物学—动物学] S965.211[农业科学—水产养殖]