壳聚糖用于毛细管电泳拆分色氨酸对映体的研究  

Enantioseparation of DL-tryptophan by Capillary Electrophoresis with Chitosan as Chiral Additive

作　　者：黄萍[1] 陈利丁[1] 姚传义[1] 

机构地区：[1]厦门大学化学化工学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2008年第4期611-613,共3页Journal of Xiamen University：Natural Science

基　　金：福建省青年科技人才创新基金(2004J018);福建省自然科学基金(E0610030)资助

摘　　要：以壳聚糖为手性添加剂,利用毛细管电泳对色氨酸对映体进行拆分,考察了壳聚糖浓度、缓冲液的pH和Cu2+浓度对分离过程的影响.实验结果表明,在壳聚糖的质量分数为0.02%、缓冲液的pH为3.5、Cu2+浓度为2mmol/L条件下,色氨酸对映体分离效果良好,分离因子为4.95,分离度为1.6.To establish a capillary electrophoresis （CE） method for the separation of tryptophan enantiomers,chitosan,a cationic natural polymer,was employed as a chiral additive selector. The separation was performed in untreated fused-silica capillaries using a Model CL1030 capillary electrophoresis system. Chitosan solution, ca. 0.01%- 0.20 % in 1% aqueous acetic acid was prepared as running buffer. As a complexing ion,Cu（ Ⅱ ） ion concentration in the buffer was varied from 1 mmol/L to 4 mmol/L. The effects of the concentration of chitosan and Cu^2＋ , pH of the buffer were investigated. A best separation of tryptophan enantiomers was achieved by using 0.02% chitosan（by weight） and 2 mmol/L Cu^2＋ while the pH of the buffer was 3, 5. The separation factor was 4. 95 and the resolution was 1.6.

关 键 词：壳聚糖 毛细管电泳 手性分离 色氨酸对映体 

分 类 号：O63[理学—高分子化学] O65[理学—化学]