带骨膜软骨与不带骨膜软骨自体异位移植的实验研究  

The experimental study on the autograft cartilages with periosteum and without periosteum

作　　者：张治家[1] 章庆国 张娇[1] 

机构地区：[1]东南大学临床医学院整形外科,江苏南京210009 [2]中国医学科学院附属协和整形医院,北京100041

出　　处：《中国美容医学》2008年第6期864-866,共3页Chinese Journal of Aesthetic Medicine

摘　　要：目的:通过软骨及带软骨膜软骨在未成年兔中的自体异位移植后变化的研究,为临床选择合适的移植物提供依据。方法:4周家兔12只,将兔的七、八、九肋软骨带骨膜取出,分别分为等长两段。一段保留骨膜,一段去除骨膜,分别埋置于兔的背部两侧对称部位。分别于4、8、12、16周分别取出所埋置的不同组的软骨。将软骨分别观察大体形态、测量长度、作组织学切片行HE染色及电镜观察其显微结构的变化。结果:4周时带骨膜软骨与周围组织结合较去骨膜软骨更紧密,16周与周围组织结合均紧密,两者无明显差异;4周时,两组软骨的长度无显著性差异,8周以后,带骨膜组较不带骨膜组更长(P<0.05);4～8周两者均增生活跃,带骨膜比不带骨膜的增生更明显;12周后软骨纤维化明显,增生不明显。结论:幼兔中,带骨膜的软骨较不带骨膜的更适于自体移植。Objective To study the changes of the cartilage with and without the periosteum heterotopic transplantation in baby rabbit, in order to choose a suitable graft for clinical transplantation. Methods 12 baby rabbits at the age of four weeks, each of them the seventh, eighth and ninth costal cartilages with periosteum were removed, and divided into two segments at the even length, one segment with periosteum the other non-periosteum. They were buried under the skin of the back at bilaterally symmetrical parts. The different group of cartilages were took out at 4,8,12,16 weeks at random. The morphology of cartilage were observed and their length were measured. Their HE staining histological sections, and microstructure under the electron microscopy were observed. Results The cartilages with periosteum integrate tightly with surrounding tissue at forth week while the cartilages without periosteum integrate tightly with surrounding tissue at sixteenth week and there was no significant difference at that time. The cells of cartilage growth actively at forth and eighth week, and those with periosteum were more actively than that without periosteaI.They growthed not so actively and had more cartilage fibrosis after 12 weeks. Conclusion The cartilage with periosteum had more advantage than that without periosteum in autologous transplantation in baby rabbits.

关 键 词：自体移植 骨膜 软骨 

分 类 号：R622[医药卫生—整形外科]