中国钩端螺旋体 rRNA 基因酶切片段长度多态性(RFLP)分析  

Characterization of Chinese Leptospiraceae by rRNA restriction fragments length polymorphisms

作　　者：李春好[1,2] 秦进才[1,2] 时曼华[1,2] 王岳竹 聂一新[1,2] 

机构地区：[1]中国预防医学科学院流行病学微生物学研究所 [2]中国药品生物制品检定所

出　　处：《中华微生物学和免疫学杂志》1997年第3期187-191,共5页Chinese Journal of Microbiology and Immunology

摘　　要：用限制性内切酶ＥｃｏＲⅠ对５６株国内外钩体参考株和２８株野生株进行ｒＲＮＡ基因ＲＦＬＰ分析时发现：８４个菌株中共有６６个不同的核糖核酸型（ＲＴ），除Ｂａｌｕｍ和Ｇｕａｎｇｄｏｎｇ型相同；赛尔东尼群的Ｈａｉｎａｎ和Ｗｈｉｔｃｏｍｂｉ型相同；赛罗群的Ｗｏｌｆｉ和Ｓａｘｋｏｅｂｉｎｇ型相同、爪哇群中的Ｍｅｎｇｒｕｎ和Ｍｅｎｇｍａ型相同外，其它的血清型都有互不相同ＲＴ。该方法和ＤＮＡ同源性研究相吻合，同一个基因型往往拥有共同的核心片段。在所检测的中国菌株中致病性钩体至少有５个基因种，腐生菌一个基因种，细丝体一个基因种。研究中发现在我国部分新的血清型菌株以及野生株中可能存在新的基因种，尤其在流感伤寒、黄疸出血、爪哇以及七日热等血清群中可能性更大。Fifty six Leptospira reference strains and 28 field isolates were examined using EcoRⅠ restriction fragments length polymorphisms of rRNA gene,sixty six leptospiral ribotypes were described.Except for strains of serovars Ballum and Guangdong giving pattern 40,Hainan and Whitcombi giving pattern 47,Wolffi and Saxkoebing giving pattern 34,Mengrun and Mengma giving pattern 66,other serovars gave specific patterns.A good correlation between DNA homology data and ribotype was observed.Furthermore,the definition of core fragments of rRNA hybridization in each recognized DNA hybridization group provided that there were five genetic species in experimental pathogenic leptospira and two genetic species in non pathogenic leptospira.The new RTs found in Chinese strains of leptospires indicated that new genetic species maybe existed in Chinese strains,especially in Icterohaemorrhagiae,Grippotyphosa,Javanica and Hebedomids serogroups.

关 键 词：钩端螺旋体 核糖核酸 基因 限制性片段长度 

分 类 号：R514.404[医药卫生—内科学] R377.5[医药卫生—临床医学]