系统性红斑狼疮患者外周血单一核细胞CD23的表达  

Study of Expression of CD23 on Peripheral Blood Mononuclear Cells in Patients with Systemic Lupus Erythematosus

作　　者：李希清[1] 凌绍贤[1] 王斌 

机构地区：[1]中山医科大学孙逸仙纪念医院皮肤科 [2]中山医科大学免疫教研室

出　　处：《中华皮肤科杂志》1997年第5期324-326,共3页Chinese Journal of Dermatology

摘　　要：为了揭示Ｂ细胞中ＣＤ２３的表达与ＳＬＥ发生发展的关系及在ＳＬＥ发病机理中可能的作用，我们应用ＡＢＣ免疫组化法和斑点核酸杂交技术对ＳＬＥ患者外周血单一核细胞（ＰＢＭＣ）ＣＤ２３蛋白和ｍＲＮＡ表达进行了检测。结果显示：３０例ＳＬＥ患者ＰＢＭＣＣＤ２３蛋白表达显著增高（Ｐ＜０．０１），且与疾病活动呈正相关关系（ｒｓ＝０．３８１４，Ｐ＜０．０５）；具有不同ＡＮＡ、抗ｄｓＤＮＡ抗体水平，有无伴肾损、脑损的ＳＬＥ患者，ＰＢＭＣＣＤ２３表达均无显著性差异（Ｐ均＞０．０５）；单纯使用皮质类固醇激素治疗或和其它免疫抑制剂联合治疗的ＳＬＥ患者，ＰＢＭＣＣＤ２３表达亦无显著性差异（Ｐ＞０．０５）。２０例ＳＬＥ患者ＰＢＭＣＣＤ２３ｍＲＮＡ表达较正常人显著增高（Ｐ＜０．０１）。经治疗病情稳定后，ＣＤ２３蛋白和ｍＲＮＡ表达均降至正常（Ｐ均＞０．０５）。提示在ＳＬＥ活动期Ｂ细胞高度激活、增殖并大量表达ＣＤ２３，且该种表达与ＡＮＡ。In order to study the relationship between expression of CD23 on B cells and the deve lopment of SLE,and possible role in the pathogenesis of SLE, we used ABC immunohistochemistry and Northern dot blot hybridization technique to detect CD23 protein and mRNA expression on PBMC in SLE patients and healthy controls.The results showed:CD23 protein and mRNA expression was significantly higher in active SLE patients than those in controls(P<0 01), and showed positive linear correlation between CD23 expression and disease activity(r s=0 3814,P<0 05), but there was no significant difference between the patients after treatment in comparison with controls(P>0 05). There were no significant difference(P>0 05) in the expression of CD23 protein on PBMC among active SLE patients with different levels of ANA or dsDNA antibody, with or without renal or brain involvment as well as treated with corticosteroids and/or other immunosuppressants. It was implicated that abnormal activation and proliferation of B cells lead to over expression of CD23 protein and mRNA in active SLE, but there was not direct relationship between over expression of CD23 and the levels of ANA or anti dsDNA antibody.

关 键 词：红斑狼疮 系统性红斑狼疮 CD23 

分 类 号：R593.241[医药卫生—内科学]