抗-HBs基因水平的研究  

Study of anti HBs on the gene level

作　　者：张春英[1,2] 仝文斌[1,2] 冯百芳[1,2] 王琰 陶其敏 

机构地区：[1]北京医科大学人民医院肝病研究所 [2]解放军海军总医院

出　　处：《中华微生物学和免疫学杂志》1997年第4期284-287,共4页Chinese Journal of Microbiology and Immunology

基　　金：卫生部科研基金

摘　　要：本研究应用ＲＴ－ＰＣＲ、ＤＮＡ－ＰＣＲ及Ｓｏｕｔｈｅｒｎｂｌｏｔ等分子生物学技术检测不同人群中针对一株抗－ＨＢｓ优势克隆的基因水平。发现慢性乙肝（ＨＢｓＡｇ阳性）患者中检出率为２０．６９％（６／２９），明显低于乙肝疫苗注射后人群的６２．９６％（１７／２７），Ｐ＜０．０１；而与未接种过乙肝疫苗组的检出率相比则无明显差异（Ｐ＞０．０５），后者阳性率为２１．４２％。这一结果提示：慢性乙肝患者与乙肝疫苗注射后相比，在抗－ＨＢｓ基因水平上存在差异，可能有抗－ＨＢｓ克隆的缺失或转录、ｍＲＮＡ翻译合成抗－ＨＢｓ的异常。本工作中对人群中抗－ＨＢｓ基因研究的尝试，为乙肝免疫耐受的研究提供了新的线索，同时也为乙肝的基因治疗提供了一定依据。One anti HBs variable region gene in different groups was determined by RT PCR,PCR and Southern blot technique.The results showed that the positive rate of the anti HBs gene was 20 69%(6/29) in patients with chronic hepatitis B,21 42%(3/14) in the group uninoculated with HB vaccine and 62 96%(17/27) in the group inoculated with HB vaccine.The rate in patients group was obviously lower than that in inoculation groups( P <0 01) and had no difference with uninoculation group ( P >0 05).The data was indicated that the patients with chronic hepatitis B existed abnormality in anti HBs genes,they might have the deletion of anti HBs clones or abnormality of anti HBs gene transcription and anti HBs antibody synthesis.

关 键 词：抗-HBs基因 免疫耐受 聚合酶链反应 

分 类 号：R512.620.3[医药卫生—内科学]