肾小球内皮细胞的凋亡诱导及ICE基因表达  

Induction of apoptosis and expression of IL 1β converting enzyme in human glomerular endothelial cells

作　　者：王建中[1,2] 陈香美[1,2] 廖洪军[1,2] 傅博 叶一舟[1,2] 于力方 

机构地区：[1]解放军总医院肾科 [2]解放军肾病中心

出　　处：《中华微生物学和免疫学杂志》1997年第5期327-329,共3页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金;优秀中青年人才基金;青年基金

摘　　要：本文首次报道用无血清培养法成功地诱导人肾小球内皮细胞凋亡，凋亡内皮细胞的ＤＮＡ电泳呈典型的“梯形结构”条带；同时用ＲＴ－ＰＣＲ发现，凋亡内皮细胞出现了明显的白细胞介素－１β转化酶（ＩＣＥ）基因表达。本结果提示ＩＣＥ基因可能在肾小球内皮细胞的凋亡过程中发挥作用，为研究细胞增殖性肾小球疾病的发生和转归提供了实验依据，也为大血管内皮细胞凋亡机制的研究增添了内容。Apoptosis was successfully induced for the first time in human glomerular endothelial cells(GECs) with the method of serum free culture without growth stimulating factors.The extracted DNA of serum free group displayed the typical “ladder pattern” indicating oligonucleosomal DNA fragmentation.By means of RT PCR,we also found apparently positive expression of IL 1β converting enzyme (ICE) gene in GECs.These results showed that serum free culture could induce apoptosis of GECs in which ICE gene might be involved.

关 键 词：肾小球内皮细胞 细胞凋亡 基因表达 ICE 

分 类 号：R692.602[医药卫生—泌尿科学]