基因转染法建立小鼠胸腺基质细胞系  

Establishment of mouse thymic stromal cell lines by gene transfection

作　　者：裴丽红[1] 余青[1] 陈慰峰[1] 

机构地区：[1]北京医科大学免疫学系

出　　处：《中华微生物学和免疫学杂志》1997年第5期330-334,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金

摘　　要：利用基因转染方法使细胞获得不死性，快速建立小鼠胸腺基质细胞（ＭＴＳＣ）系。用含有多瘤病毒ｍＴ基因的重组质粒Ｚｉｐ－ｍＴ以脂质体介导法转染初代培养的ＭＴＳＣ，经过８００μｇ／ｍｌ的Ｇ４１８筛选，得到５个ＭＴＳＣ克隆。Ｓｏｕｔｈｅｒｎ杂交分析表明所得细胞克隆的染色体中整合有ｍＴ基因，说明ｍＴ基因的整合是导致细胞转化的原因。基因转染建系比常规建系所需时间大大缩短，仅需２个月。For rapid establishment of mouse thymic stromal cell(MTSC) lines,gene transfection was used to immortalize fresh viable cells.Primary MTSC cultures were transfected with Zip mT(harbouring polyoma virus middle T gene)together with lipofectin.After selection by G418 up to 800μg/ml,5 MTSC clones have been obtained.Southern blot hybridization analysis indicated that the immortalization of the MTSC clones was attributed to the insertion of mT genes.Through gene transfection,MTSC clones can be established in two month time,much shorter than that of conventional methods.

关 键 词：基因转染 胸腺基质细胞 多瘤病毒 mT基因 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学]