表达CS3菌毛和CT-B抗原双价疫苗候选株的构建  

Construction of bivalent vaccine candidate expressing CS3 fimbriae and cholera toxin B subunit

作　　者：董自正[1] 李淑琴[1] 张兆山[1] 黄翠芬[1] 

机构地区：[1]军事医学科学院生物工程研究所

出　　处：《中华微生物学和免疫学杂志》1997年第5期348-351,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家科委"863"计划

摘　　要：肠毒素性大肠杆菌（ＥｎｔｅｒｏｔｏｘｉｇｅｎｉｃＥｓｃｈｅｒｉｃｈｉａｃｏｌｉ，ＥＴＥＣ）是引起幼儿腹泻和旅游者腹泻的重要致病菌。本研究的目的是构建既具有抗菌能力又有中和毒素能力的工程菌株。本研究以Ｂｌｕｅ－ｓｃｒｉｐｔ质粒为载体构建了表达ＣＳ３和ＣＴ－Ｂ的双价重组抗原的克隆株。口服免疫ＢＡＬＢ／ｃ小鼠和肌肉注射免疫家兔，该重组菌株可以诱发抗ＣＴ－Ｂ和抗ＣＳ３的双重免疫应答，为构建多价腹泻疫苗株奠定了基础。Enterotoxigenic Escherichia coli (ETEC) is one of the most important pathogens of diarrhea in children under age 5 years and in travelers.A multicomponent vaccine made up of anti colonization factor immunity plus toxoids may provide the best immune stimulation.A recombinant strain which could express bivalent antigens of CS3 fimbriae and cholera toxin B subunit (CT B) was constructed using plasmid vector bluescript.Inoculating mice orally and immunizing rabbits intramuscularly demonstrated that the recombinant strain could induce immune response against the two antigens.

关 键 词：肠毒素大肠杆菌 CS3菌毛 基因重组疫苗 

分 类 号：R392.7[医药卫生—免疫学]