分泌性中耳炎中耳积液中肿瘤坏死因子-α的检测  

DETECTION OF TUMOR NECROSIS FACTOR-ALPHA IN MIDDLE EAR EFFUSION OF OTITIS MEDIA WITH EFFUSION

作　　者：郭敛容[1] 卢永德[1] 田丰[1] 任基浩[1] 

机构地区：[1]湖南医科大学附二院耳鼻咽喉科

出　　处：《中国耳鼻咽喉颅底外科杂志》1997年第3期167-169,共3页Chinese Journal of Otorhinolaryngology-skull Base Surgery

摘　　要：目的：为探讨分泌性中耳炎（ＯＭＥ）患者中耳积液（ＭＥＥ）中肿瘤坏死因子－α（ＴＮＦα）的存在及其在ＯＭＥ发生中的作用。方法：运用酶联免疫吸附法（ＥＬＩＳＡ）检测３３例ＯＭＥ患者ＭＥＥ及血清中ＴＮＦα的浓度。结果：ＭＥＥ中ＴＮＦα的含量明显高于对应血清中含量（Ｐ＜０００１）；粘液组ＭＥＥ中ＴＮＦα的含量明显高于浆液组ＭＥＥ中的含量（Ｐ＜０００１）；儿童组中耳积液ＴＮＦα水平明显高于成人组（Ｐ＜０００１）。结论：中耳局部可产生ＴＮＦα，ＴＮＦα可能与ＭＥＥ的形成有关；粘液性中耳炎ＴＮＦα明显增高，反映其局部炎症程度较浆液性中耳炎重；儿童、成人ＭＥＥ中ＴＮＦα的水平差异表明发病机理可能有所差别。In order to inverstigate the role of tumor necrosis factor alpha (TNFα) in the production of middle ear effusion (MEE), the levels of TNFα in MEE and sera were studied of 33 patients sufferring from otitis media with effusion (OME) by using an enzyme linked immunosorbent assay system. The results show that the level of TNFα in MEE was significanly higher than that in sera ( P <0 001); the level of TNFα in mucoid otitis media was higher than that of serous otitis media ( P <0.001); the level of TNFα in MEE was higher in children than that in adults ( P <0.001). It is concluded that TNFα can be produced by middle ear mucosa and TNFα may attribute to the production of MEE. The local infection of mucoid otitis is more severe than that of serous otitis. The level of TNFα in MEE of child group was higher than that of adult group, which indicates that the pathogenesis of MEE may be different.

关 键 词：肿瘤坏死因子Α 分泌性 中耳炎 中耳积液 

分 类 号：R764.210.4[医药卫生—耳鼻咽喉科]