Magnaporthe oryzae MTP1 gene encodes a type Ⅲ transmembrane protein involved in conidiation and conidial germination  

作　　者：Qin LU Jian-ping LU Xiao-dong LI Xiao-hong LIU Hang MIN Fu-cheng LIN 

机构地区：[1]College of Life Sciences, Zhejiang University, Hangzhou 310058, China [2]Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2008年第7期511-519,共9页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：the National Natural Science Foundation of China (Nos. 30671351 and 30470064);the Natural Science Foun-dation of Zhejiang Province, China (No. Y304211)

摘　　要：In this study the MTP1 gene, encoding a type III integral transmembrane protein, was isolated from the rice blast fungus Magnaporthe oryzae. The Mtp 1 protein is 520 amino acids long and is comparable to the Ytp 1 protein of Saccharomyces cerevisiae with 46% sequence similarity. Prediction programs and MTP1-GFP （green fluorescent protein） fusion expression results indicate that Mtp 1 is a protein located at several membranes in the cytoplasm. The functions of the MTP1 gene in the growth and development of the fungus were studied using an MTP1 gene knockout mutant. The MTP1 gene was primarily expressed at the hyphal and conidial stages and is necessary for conidiation and conidial germination, but is not required for pathogenicity. The Amtpl mutant grew more efficiently than the wild type strain on non-fermentable carbon sources, implying that the MTP1 gene has a unique role in respiratory growth and carbon source use.In this study the MTP1 gene, encoding a type Ⅲ integral transmembrane protein, was isolated from the rice blast fungus Magnaporthe oryzae. The Mtp1 protein is 520 amino acids long and is comparable to the Ytp1 protein of Saccharomyces cerevisiae with 46% sequence similarity. Prediction programs and MTP1-GFP (green fluorescent protein) fusion expression results indicate that Mtp1 is a protein located at several membranes in the cytoplasm. The functions of the MTP1 gene in the growth and development of the fungus were studied using an MTP1 gene knockout mutant. The MTP1 gene was primarily ex-pressed at the hyphal and conidial stages and is necessary for conidiation and conidial germination, but is not required for patho-genicity. The Δmtp1 mutant grew more efficiently than the wild type strain on non-fermentable carbon sources, implying that the MTP1 gene has a unique role in respiratory growth and carbon source use.

关 键 词：Magnaporthe oryzae Magnaporthe grisea MTP1 CDNA Gene knockout Conidial germination 

分 类 号：Q78[生物学—分子生物学]