血管紧张素Ⅱ受体阻滞剂联合放射对鼻咽癌细胞增殖和侵袭能力的影响  

The inhibitory effect of angiotensin Ⅱ type 1 receptor blocker combined with radiation on the proliferation and invasion ablility of human nasopharyngeal carcinoma cells

作　　者：王琼[1] 赵伟[1] 李贵玲[1] 张盛[1] 伍钢[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院肿瘤中心,武汉430023

出　　处：《中华放射肿瘤学杂志》2008年第4期282-285,共4页Chinese Journal of Radiation Oncology

摘　　要：目的研究血管紧张素Ⅱ受体（AT1R）阻滞剂缬沙坦对鼻咽癌细胞系（CNE-2）是否具有放射增敏作用，以及它与放射联合对CNE-2细胞侵袭能力的影响。方法用成克隆分析法观察缬沙坦与放射联合对CNE-2细胞体外存活效应的影响，用流式细胞术和体外侵袭实验（小室法）检测对细胞凋亡和侵袭能力的影响。结果CNE-2细胞经10^-9、10^8、10^-7 mol／L缬沙坦与放射联合作用后，放射增敏比（SER）分别为1．10、1．20、1．36；侵袭能力抑制率分别为8．11％、16．49％、16．77％。改变缬沙坦作用时间，SER随作用时间延长而增加。经10^-8 mol／L缬沙坦和放射处理24h后，CNE-2细胞凋亡率为1．89％±0．09％，与单纯放射组1．62％±0．06％相比有所提高（t=4．79，P〈0．05）。结论AT1R阻滞剂缬沙坦在体外对CNE-2细胞有放射增敏作用，与放射联合能抑制细胞侵袭能力和在一定程度诱导细胞凋亡，为缬沙坦体内实验的放射增敏效应提供基础。Objective To investigate the effect of valsartan, an angiotensin Ⅱ type 1 receptor （AT1 R） blocker, on radiosensitivity, invasive potential and proliferation activity of nasopharyngeal carcinoma cells（CNE-2） in vitro. Methods Radiosensitization of valsartan on CNE-2 cells in vitro was investigated by colony forming assay. Effect of AT1R blocker combined with radiation on invasive potential of CNE-2 cells was evaluated using 24-well Matrigel invasion chambers （Transwell）. Apoptosis-inducing effect of valsartan combined with radiation on apoptosis of CNE-2 was identified by flowcytometry （ FCM ）. Results When valsartan was given at 10^-9, 10^-8 and 10^-7 mol/L combined with radiation, sensitivity enhancement ratios （SER） were 1.10,1.20 and 1.36 ,and the invasive inhibition rates were 8.11% , 16.49% and 16.77% ,respectively. The SER of valsartan on CNE-2 distinctly increased when the exposure time was increased. After 24 h exposure to 10^-8 mol/L valsartan combined with radiation, the apoptosis rate was 1.89% ± 0.09% , which was higher than 1.62% ± 0.06% in radiation alone group （ t = 4.79, P 〈 0.05 ）. Conclusions AT1R blocker valsartan combined with radiation can significantly inhibit the proliferation activity of nasopharyngeal carcinoma cells in vitro in a dose- and time-dependent manner. Valsartan combined with radiation can potently inhibit the invasive potential of CNE-2, which may be involved in the mechanism of valsartan treatment in vivo.

关 键 词：细胞系 鼻咽癌 放射增敏剂 凋亡 侵袭 

分 类 号：R686[医药卫生—骨科学]