药物在大鼠主动脉组织及其培养细胞的细胞膜色谱研究  

Chromatographic study on bio-affinity of drugs to rat aorta tissue and cultured smooth muscle cell in cell membrane

作　　者：张典[1] 袁秉祥[1] 邓秀玲[1] 杨广德[2] 贺浪冲[2] 

机构地区：[1]西安交通大学医学院药理学系 [2]西安交通大学药学系,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2008年第3期274-276,280,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30171079)

摘　　要：目的比较细胞膜色谱法中细胞膜固定相的特异性。方法分别构建大鼠主动脉组织匀浆细胞膜固定相和培养大鼠主动脉平滑肌细胞膜固定相。用细胞膜色谱法研究10种-αAR激动剂和拮抗剂的生物亲和作用,测定其容量因子并进行相关性分析。结果10种药物在大鼠主动脉组织和培养大鼠主动脉平滑肌细胞膜固定相上容量因子之间存在正相关关系,相关系数r=0.923,有极显著性意义(P<0.01)。结论用培养的细胞制备细胞膜固定相具有更高的特异性,可提高药物的保留时间,也可用于药物的高效初筛。Objective To compare the specificities of the cell membrane stationary phases （CMSP） with cell membrane chromatography （CMC）. Methods Cell chromatographic columns were constructed for both rat aorta tissue cells and cultured rat aorta smooth muscle cells. Then the chromatographic affinities of ten ligands of α-adrenergic receptor （α AR） with both said chromatographic columns were investigated. Capacity factors （k＇）, as a chromatographic parameter, were calculated. Results The correlation analysis showed a positive correlation between the rat aorta tissue CMSP and the cultured rat aorta smooth muscle cell CMSP, with correlation factor of r = 0. 923, P〈0.01. Conclusion By improving the specificity with the CMSP of cultured rat aorta smooth muscle cell, the enhanced CMC method is capable of affinities for evaluating ligand-receptors or drug screening.

关 键 词：细胞膜 色谱 Α-肾上腺素受体 

分 类 号：R967[医药卫生—药理学]