肝舒汤对非酒精性脂肪肝大鼠脂质过氧化的影响  

Anti-peroxidation effects of Ganshutang decoction on experimental rats with non-alcoholic fatty liver disease

作　　者：杨成志[1] 狄灵[1] 聂丹丽[1] 于燕[2] 阮班云[1] 

机构地区：[1]西安交通大学医学院第二附属医院中西医结合科,陕西西安710004 [2]西安交通大学医学院公共卫生系,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2008年第3期336-339,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省科技攻关资助项目(No.2005K16-G5(4))

摘　　要：目的探讨中药复方肝舒汤对非酒精性脂肪性肝病(NAFLD)模型大鼠脂质过氧化反应的影响,探讨其防治NAFLD的作用机制。方法采用高脂饮食复制大鼠NAFLD模型。随机分为正常组、模型组、中药组、东宝肝泰组。检测血脂变化、肝匀浆超氧化物歧化酶(SOD)活性、丙二醛(MDA)及观察肝细胞超微结构。结果模型组大鼠出现脂肪变及不同程度的炎性细胞浸润,电镜可见肝细胞内存在大量脂滴,模型组大鼠血和肝组织中MDA水平较正常组显著升高,而SOD活性则较正常组显著降低,与模型组相比中药组大鼠血清TG、LDL-c明显降低;肝匀浆中MDA含量显著降低,肝细胞超微结构明显改善。结论肝舒汤对大鼠非酒精性脂肪肝有明显的防治作用,其作用机制与其能降低血脂、改善肝脏组织脂肪变性程度、抑制脂质过氧化有关。Objective To observe the effects of Chinese herbs Ganshutang decoction on lipid peroxidation reaction in rats with non-alcoholic fatty liver （NAFL）, and inquire into its mechanism of preventing and treating NAFL. Methods Thirty-two rats were randomly allocated into 4 groups： normal control group, model group, Dongbao Gantai （DBG） group and Ganshutang decoction （GSTD） group. The model of NAFLD in SD rats was induced by a term of 8-week high fat diet. General condition, pathological changes of liver tissues and the ultrastructure of liver cell were observed. Blood lipids were detected. The levels of MDA and SOD of liver tissue were also measured. Results Compared with that of the normal group, the liver tissue developed steatosis and gradually infiltrated with inflammation cells, and necrosis appeared in hepatocytes. The liver ultrastructure indicated that there were many lipid droplets accumulated in the plasm of model hepatocytes. The level of MDA in liver tissue of the model group obviously increased, while the activity levels of SOD obviously decreased. Compared with that of model group, GSTD could improve the liver pathologic structure, and decrease blood lipids and liver tissue MDA significantly. Conclusion Ganshutang decoction can prevent and treat NAFL obviously, the mechanism of which may be related to preventing lipid peroxidation reaction.

关 键 词：非酒精性脂肪肝 肝舒汤 氧化应激 

分 类 号：R285.5[医药卫生—中药学]