广西地区动物HEV基因型及亚型分布  

Study on genotypes and subgenotypes of hepatitis E virus among animals in Guangxi

作　　者：韦献飞[1] 吉彦莉[2] 唐荣兰[1] 泰小云[1] 梁靖瑞[2] 李凌君[2] 葛宪民 熊云新[1] 谢益安[1] 朱永红[2] 

机构地区：[1]柳州医学高等专科学校公共卫生系,柳州545006 [2]北京大学医学部病原生物学系 [3]广西职业病研究所

出　　处：《中国公共卫生》2008年第7期778-780,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金项目(30570063);国家“863”基金项目(2006AA02Z453);广西科学基金项目(桂科自:0728242)

摘　　要：目的了解广西地区猪、鼠、狗、猕猴和鱼5种动物戌型肝炎病毒（HEV）基因型和亚型分布。方法对广西地区猪、鼠、狗HEV抗体阳性血清170份,鼠肝组织150份,3月龄以下狗粪便标本80份,鱼胆汁标本120份,3月龄以下猪粪便标本129份,龙虎山自然景区猴粪便标本130份,用巢式逆转录聚合酶链反应法（RT-nPCR）检测戊型肝炎病毒核糖核酸（HEV RNA）,对RT-nPCR阳性扩增产物进行克隆测序,用Vector NTI Suite 9.0和Treeview软件与目前新分型方法所选参考序列进行核苷酸序列相似性比较和生物进化树分析。结果广西地区170份猪、鼠和狗血清HEV RNA检测均为阴性。在150份鼠肝组织,120份鱼胆汁标本,80份狗和130份猕猴粪便标本中均未检出HEV RNA;3月龄以下猪粪便标本HEV RNA阳性率10.08（13/129）。对13株猪HEV开放读码框架（ORF）1区部分核酸序列进行分析,其与Ⅰ、Ⅱ、Ⅲ和Ⅳ型HEV的相似性分别为73%～77%,76%～79%,76%～81%,82%～97%;与6个亚型的相似分别为4a：82%～94%;4b：82%～98%;4c：82%～87%;4d：81%～87%;4f：84%～86%;4g：81%～84%,其中2株与Ⅳa亚型相似性是高为82%～94%,11株与Ⅳb亚型相似性最高为82%～98%。结论广西地区猪HEV均为基因型4,2株属于基因型Ⅳa,Ⅱ株为Ⅳb。HEV在猪、鼠、狗血清、鼠肝组织、鱼胆汁、狗和猕猴粪便标本中均未检出。Objective To detect hepatitis E virus（HEV） and its genotypes and subgenotypes among swine, rats, dogs, Rhesus Macagues and fishes in Guangxi. Methods 170 HEV antibody positive serum samples from swine, rats, and dogs, 150 liver tissue samples from rats, 80 fecal samples from dogs under the age of 3 months, 120 bile samples from fishes, 129 fecal samples from swine under the age of 3 months, and 130 fecal samples of Rhesus Macaques in Longhushan Natural Park in Guangxi were collected. HEV RNA was amplified by reverse transcription nested polymerase chain reaction （ RT - nPCR）, and RT - nPCR positive products were cloned and sequenced. The nucleotide sequence comparison and phylogenetic analysis of HEV for positive samples isolated were made by using the Vector NTI Suite 9.0 and Three View Softwares. Results The HEVRT - nPCR of all 170 serum samples from swine, rats and dogs were negative, and HEV RNA was also negative among all the remaining samples, including 150 liver tissue samples from rats, 120 bile samples from fishes, fecal samples from 80 dogs and 130 Rhesus Macaques. The HEV positive rate of fecal samples from swine under the age of 3 months was 10.08 % （13/129）. These 13 swines＇ HEV ORF1 nucleotide sequences islates shared similarities of 73 - 77 %, 76 % - 79 %, 76 %～81%, 82 % - 97 % with genotype Ⅰ - Ⅳ, respectively. Their similarities with 6 subgenotypes were 82 % ～ 94 % （4a）, 82 % 98 % （4b）, 82 % ～ 87 % （4c）, 81% ～ 87 % （4d）, 84 % ～ 86 % （4f）, 81% ～ 84 % （4g）, respectively. Among those 13 isolates, 2 isolates shared the highest similarity at 82 % ～ 94 % with Ⅳa and 11 isolates shared the highest similarity at 82 % ～ 98 % with IVb. Conclusion HEV strains of swine from Guangxi were classified as HEV genotype Ⅳ, 2 of which were classified as HEV IVa subgenotype and 11 of which were classified as HEV IVb subgenotype. HEV was not detected positively in all serum samples from swine, rats and dogs, lver tissue from rats, fecal samples from dogs an

关 键 词：戊型肝炎病毒 基因型 亚型 动物宿主 

分 类 号：R373.2[医药卫生—病原生物学]