医院金黄色葡萄球菌耐药及质粒同源性分析  

Drug-resistance and plasmid homology analysis of SA in medical environment

作　　者：樊景春[1] 白亚娜[2] 石福艳[1] 裴泓波[2] 

机构地区：[1]潍坊医学院预防医学系卫生统计学教研室,山东潍坊261042 [2]兰州大学公共卫生学院流行病与卫生统计学研究所

出　　处：《中国公共卫生》2008年第7期816-817,共2页Chinese Journal of Public Health

基　　金：甘肃省自然科学基金项目(EZ031-A25-060-E)

摘　　要：目的了解医院环境金黄色葡萄球菌耐药性及质粒同源性,为制定预防外源性医院感染措施提供依据。方法抽取2所省级医院5个科室进行消毒前随机采样,进行细菌培养分离鉴定,并对金黄色葡萄球菌进行药敏实验,质粒消除、质粒图谱及限制性内切酶图谱分析。结果196份样本中,分离到金黄色葡萄球菌15株,占7.7%;其中空气检出率最高,为19.1%。金黄色葡萄球菌的青霉素耐药率为60.0%,3种或以上耐药46.7%;有12株（80.0%）检出质粒,11株含质粒的耐药金葡菌进行质粒消除后,有9株耐药性变化,2株未变化。耐药质粒分子量1.06～39.93kb。3株含有共同耐药谱的乙医院烧伤科金葡菌有相似质粒,不同医院也有相似质粒谱。用EcoRI酶切4株金葡菌质粒后,质粒片段增多,分子量变化,含有5种相同大小的质粒。结论金葡菌的高危传播途径是空气且耐药性高,不同医院和不同科室的金葡菌有同源耐药质粒。Objective To know drug - resistance and plasmid homology of SA in medical environment and provide data for controlling nosocomial infection in hospital environment. Methods Five wards from two provincial hospitals in Lanzhou city were selected to collect different location samples randomly. IsoLating, culturing, identifying and testing susceptibility of SA, plasmid elimination, plasmid profile and restriction endonuclease profile were used to analyze the homology. Results 15 （7.7 % ） SA were isohted from 196 samples, the highest SA positive rate is air （19.1% ）. 60.0 % of SA isolated was resistant to PG and 46.7 % was resistant to more than three antibiotics. 12 （80.0 % ） strains of SA have plasmids between 1.06- 39.93kb, 3 strains have same resistance profile and similar plasmid profile from hospital B. Restriction endonudease profile shows 4 strains have 5 same kinds of plasmid. Conclusion Air is the SA high risk transmit pathway and drug resistance is serious, homological drug- resistant plasmid exists in SA strains sampled from different hospitals and different wards.

关 键 词：金黄色葡萄球菌 医疗环境 耐药 质粒 同源性 

分 类 号：R378.11[医药卫生—病原生物学] R978.11[医药卫生—基础医学]