高效液相色谱法测定调经活血片中芍药苷的含量  

Determination of paeoniflorin in Tiaojing Huoxue Tablets by HPLC

作　　者：王玉鹏[1] 赵丽华[1] 王杰松[2] 

机构地区：[1]武警安徽总队医院药剂科,合肥230041 [2]解放军第306医院药学部,北京100101

出　　处：《武警医学》2008年第7期617-619,共3页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的建立HPLC法测定中成药调经活血片中芍药苷含量。方法用Waters C18色谱柱。流动相为乙腈-0．05mol／L磷酸二氢钾溶液（15：85），流速为1．0mL／min，检测波长为230nm，柱温为25℃。结果芍药苷在0．01～0．50mg／ml浓度范围内与峰面积呈良好的线性关系（r=0．9999），平均回收率为98．84％，RSD为1．37％（n=6）。结论该方法准确，灵敏度高，重现性好，可作为调经活血片的质量控制指标。Objective To estabhsh an HPLC method for determination of paeoniflorin in Tiaojing Huoxue Tablets.Methods A Waters C18 column （250 mm×4.6mm, i.d. 5μm） was used, with mobile phase of acetonitrile 0.05 mol and L KH2PO4（15：85）, a flow rate of 1.0 ml/min, detection wavelength at 230 nm, and column temperature at 25℃. Results Paeoniflorin concentration in the range of 0.01-0.50 mg/ml was linear with peak area （ r =0.9999）. Tbe mean recovery was 98.84%,RSD=1.37% （n=6）.Conclusions method is sensitive,accurate,and reproducible;it can be used as the quality control index for Tiaojing Huoxue Tablets.

关 键 词：调经活血片 芍药苷 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]