猪肝线粒体F_1-ATP合酶的提取及检测方法  

Extraction and Detection of F_1-ATP Synthase from Pig Liver Mitochondria

作　　者：刘田[1] 朱华泽[1] 徐平平[1] 

机构地区：[1]陕西师范大学物理学与信息技术学院,陕西西安710062

出　　处：《陕西师范大学学报（自然科学版）》2008年第4期37-39,共3页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(20772077);教育部科学技术重点研究资助项目(104167)

摘　　要：采用超声和差速离心方法,研究了猪肝线粒体ATP合酶水溶性部分(F1-ATP合酶)的提取及检测方法.对初始提取液和纯化后的溶液分别进行酶比活力测量,并对常规聚丙烯酰胺电泳后的胶条进行胶内活性检测.结果表明,纯化后的F1-ATP合酶水解ATP比活力显著提高.The extraction and detection of F1-ATP synthase from pig-liver mitochondria are investegated by using ultrasonic and differential centrifugal methods. The specific activity of F1- ATP synthase in both primary and purified solutions are measured first, then the in-gel enzymatic anylysis are made after polyacrylamide gel electrophoresis. The results show that the specific activity of F1-ATP synthase in purified solution is increased compared with that of the primary solution.

关 键 词：F1-ATP合酶 差速离心 超声 聚丙烯酰胺电泳 

分 类 号：Q945.11[生物学—植物学] Q786