一株对新月弯孢霉等香蕉病原真菌具有良好拮抗作用的菊欧氏杆菌  

An Strain of Erwinia chrysanthemi with Good Antagonisticity against Curvularia Leaf Spot Pathogen of Banana

作　　者：殷晓敏[1] 陈弟[1] 郑服丛[2] 

机构地区：[1]海南大学环境与植物保护学院,海南儋州571737 [2]中国热带农业科学院环境与植物保护研究所,海南儋州571737

出　　处：《热带农业科学》2008年第3期38-40,共3页Chinese Journal of Tropical Agriculture

基　　金：中央级公益性科研院所基本科研业务专项资助项目(No.2007hzs1J011)

摘　　要：研究了香蕉内生菊欧氏杆菌(Erwinia chrysanthemi Burkholder,McFadden et Dimock)E353菌株对香蕉弯孢霉叶斑病菌新月弯孢霉[Curvularia lunata(Wakker) Boedijn]等4钟香蕉病原真菌的抑制效果。对峙培养法和孢子萌发悬滴法检测结果表明,E353菌株对新月弯孢霉具有良好抑制效果。E353培养液滤液明显抑制靶标菌菌丝扩展,形成的抑菌带宽0.6cm;E353滤液使病原真菌芽管的生长点致畸膨大成串珠状,其半致死浓度EC50为4.48%(E353滤液的体积分数)。E353滤液对香蕉枯萎病菌香蕉镰刀菌、香蕉炭疽病菌芭蕉炭疽菌、香蕉链格孢叶斑病菌番茄链格孢亦有良好的拮抗活性,其EC50分别为6.14%、4.57%、7.59%。An endophyfic strata of Erwinia chrysanthemi Burkholder, McFadden et Dimock, E353, was isolated from the pseudostem of banana, and its antagonistic action against Curvularia leaf spot （Curvularia lunata （Wakker） Boedijn） was tested by pairing culture and conidial germination of plant pathogens. The Strain E353 culture broth evidently inhibited the spread of hyphas of the target pathogenic fungi, and inhibitive zones 0.6 cm wide were observed. The fermentation broth of the strain E353 significantly inhibited conidial germination of C. lunata, and Colletotrichum musae （Berkeley et MA. Curtis） yon Arx, Fusarium oxysporum Schlechtendahl f. sp. cubense （EF. Smith） Snyder et Hansen, and Alternaria alternata （Fries） yon Keissler. Their ECso were 4.48%, 6.14%, 4.57%, 7.59% respectively. The acting mechanism of this antagonistic strain E353 showed that some cells of hyphas and spores became strings of beads.

关 键 词：生物防治 菊欧氏杆菌(菊欧文氏菌) 新月弯孢霉 香蕉弯孢霉叶斑病 拮抗 抑制 真菌病害 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S436.67[农业科学—植物保护]