中耳胆脂瘤上皮中前列腺素E_2和IL-1β的表达及临床意义  

Expression and clinical significance of PGE2 and IL-1β in human middle ear cholesteatoma epithelium

作　　者：李江民[1] 刘兆辉[1] 安伟[1] 林世龙[1] 陈天会[1] 林季珍[1] 

机构地区：[1]遵义医学院附属医院,贵州遵义563003

出　　处：《山东医药》2008年第21期20-21,共2页Shandong Medical Journal

基　　金：遵义医学院青年医师启动基金资助项目(2006-017)

摘　　要：目的探讨前列腺素(PG)E2及IL-1β在中耳胆脂瘤中表达及其相关性。方法采用酶联免疫法和放射免疫法测定胆脂瘤上皮组织匀浆液中及正常外耳道皮肤组织匀浆液中IL-1β、PGE2水平。结果在12例中耳胆脂瘤上皮及10例正常外耳道皮肤组织中,IL-1β的表达分别为(313.562±52.924)、(141.414±31.335)pg/ml,PGE2的表达分别为(1672.371±201.342)、(886.469±130.915)pg/ml(P均<0.01)。在胆脂瘤上皮中,IL-1β表达与PGE2表达呈正相关(r=0.540,P<0.05)。结论IL-1β及PGE2在中耳胆脂瘤上皮中存在高表达。IL-1β可诱导PGE2表达,两者协同促进胆脂瘤上皮增生。Objective To investigate the expression of interleukin-1 beta （IL-1β） and prostaglandin E2 （PGE2） in cholesteatoma and approach the possible role of them in the formation and development of the middle ear cholesteatoma. Methods The expression of IL-1β and PGE2 in middle ear cholesteatoma specimens and normal external ear canal skin were examined by the technology of ELISA method and RIA method. Results The values of IL-1β expression in the 12 middle ear cholesteatoma epithelium tissue samples and 10 external ear cannel＇s normal skin specimens were （313.562 ± 52.924）and（141.414±31.335）pg/ml（P〈0. 01）, and those OfPGE2 were（1 672.371 ±201.342 ）and（886.469 ± 130. 915）pg/ml（ P 〈0. 01 ）, respectively. Linear correlation analysis showed that in the cholesteatoma epithelium, IL-1β was positively correlated with PGE2 （ r = 0. 540, P 〈 0. 05 ）. Conclusions High density of IL-1β and PGE2 is found in the epithelium of cholesteatoma. IL-1β induces the expression of PGE2 protein ,and promotes cholesteatoma epithelium proliferation in coordination with PGE2. All above indicate hypoxia and its relative factors IL-1β,PGE2 play an important role in the formation and development of cholesteatoma.

关 键 词：中耳 胆脂瘤 前列腺素E2 白细胞介素-1Β 

分 类 号：R764[医药卫生—耳鼻咽喉科]