提取地衣真菌总DNA的简便方法  

A Convenient Method of Extracting Total DNA from Lichen Fungus

作　　者：时向阳[1,2,3] 魏江春[1,2,3] 姜玉梅 田素忠[1,2,3] 郭守玉 

机构地区：[1]北京林业大学森林资源与环境学院 [2]中国科学院微生物所真菌地衣系统学开放实验室 [3]北京市林业局森防站

出　　处：《北京林业大学学报》1997年第4期45-50,共6页Journal of Beijing Forestry University

基　　金：国家自然科学基金

摘　　要：利用液氮破碎地衣体的上下表皮层及藻层，再用氯化苄在弱碱条件下破坏真菌细胞壁，从而获得地衣真菌总ＤＮＡ．此方法简便、省时、价廉，获得的总ＤＮＡ蛋白质污染少，产量高，可进一步进行ＰＣＲ扩增．The process of the new method is that the upper or lower cortex and thaus of lichens are broken by Liquid Nitrogen,then fungi cell walls are destroyed by Benzyl Chloride(Phch 2Cl) under weak base condition,so the total DNA of lichen fungus is gained.The method is simple and convenient,so it can save both time and money.The gained total DNA has little miscellaneous proteins and DNA production is high.The DNA could be used for PCR amplification,RFLP analysis and sequencing analysis.

关 键 词：地衣真菌 地衣体 总DNA PCR扩增 

分 类 号：Q939.506[生物学—微生物学]