小型猪慢性心肌缺血模型四诊信息采集的方法探索  

Research on Collection of Four Diagnostic Information from Swine Model with Chronic Myocardial Ischemia

作　　者：刘蕾[1] 王伟[1] 郭淑贞[1] 许文玉[1] 于永新[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中华中医药学刊》2008年第7期1438-1440,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(2003CB517105)

摘　　要：目的：探讨中华实验小型猪慢性心肌缺血模型构建方法以及探讨其四诊信息采集方法。方法：采用中国农业大学育种健康中华小型猪，体重（25±4）kg，月龄6～10个月，雌雄不限，动物在麻醉、无茵条件下开胸，分离前降支，在第一对角支远端的前降支主干放置Ameroid缩窄环，关胸。并通过心电图、超声心动图及冠脉造影评价小型猪慢性心肌缺血模型构建成功与否。在造模过程中，参照中医的四诊信息采集办法，并参考中兽医在大动物辨证方面的经验，制定出小型猪证候观察量表，在实际操作中通过望、闻、问、切四诊方法对模型动物宏观体征进行动态观察，并借助心电图、数码照相等现代手段间接地获得四诊信息。结果：模型动物在冠脉环缩术后1—8周内，精神状态、舌象、饮食、饮水等方面均有明显变化。数码相机所拍舌象经Adobe Photoshop8．0分析所得R、G、B值与假手术组相比有显著性差异。结论：对小型猪的四诊信息采集是可行的、客观的，但更需不断发掘、完善，制定出量化标准。Objective ：To establish a swine model of chronic myocardial ischemia（CMI） and probe into collecting the four diagnostic methods of the models. Methods ： Chinese experimental mini swines （ 25±4 ） kg were instrumented with a size -matched Ameroid constrictor on the anterior descending branch under general anesthesia in sterile conditions. Basing on early results of dynamic observations, evaluation was performed four weeks after operation. Clirtieal performances of animals were collected and electrocardiogram, echocardiography, coronary angiography were detected. During the process the four diagnostic information was collected by the methods of inspection,listening and smelling, asking and pulse -taking,Meanwhile,the ECG and DC are used to deserve the information of four diagnostic methods. Results： After the operation ,there were obviously changes on mental state,tongue demonstration, food and drink, and so on. Model animals appeared mental stress ,irritation, fear, violent behaviors, strong self- defense, pilose disorderly and lack of luster, slightly dark red or purple tongue ,etc. While the sham operated animals＇performances gradually returned to normal ,with increased appetite, neat and shinypilose,light red or light white tongue. There were a significant decrease of R ratio and increase of B ratio in the model animals＇tongue color. Image of tongue dealt with Adobe Photoshop 8.0 ,the result indicated that R - ratio, G - ratio and B - ratio obviously changed after operation, compared with sham operation. Conclusion ： The approach that implantation Ameroid constrictor around the LAD in the swine was applicable to make an animal model of chronic coronary stenosis. And such models can be adapted in research of chronic myocardial ischemia of coronary heart disease and relative diseases. Since syndrome is the core theory of Chinese medicine, the revealing of its scientific connotation is considered as the key point to the modernization of Chinese medicine. However,it depends on the breakthrou

关 键 词：小型猪 慢性心肌缺血模型 四诊信息 

分 类 号：R2-03[医药卫生—中医学] R-33