用于水产品中氯霉素残留检测的发光细菌的筛选  

Screening of luminescent bacteria for detection of chloramphenicol in aquatic foods

作　　者：朱兰兰[1] 王静雪[1] 林洪[1] 王亚群[1] 王晶[1] 梅册霞[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,青岛266003

出　　处：《高技术通讯》2008年第7期754-760,共7页Chinese High Technology Letters

基　　金：国家自然科学基金(30500384);863计划(2006AA09Z431)资助项目

摘　　要：从青岛近海分离鉴定出5株发光细菌,经过16SrDNA 测序及生理生化实验鉴定,分属明亮发光杆菌(DB)、鳆发光杆菌(YL)、哈维弧菌(BA)、灿烂弧菌(GQ)、费氏弧菌(GX)。发光性质及其发光规律曲线的测定表明,鳆发光杆菌和明亮发光杆菌发光规律稳定,持续发光时间长,发光强度大。所得鳆发光杆菌、哈维弧菌、灿烂弧菌、费氏弧菌的发光规律曲线国内未见报道。进行了各菌种的氯霉素抑菌圈实验,发现5株发光细菌对氯霉素都敏感,鳆发光杆菌 YL 最敏感。用发光细菌 YL 检测了南美白对虾中的氯霉素,其前处理方法比水产行标气相色谱法简便省时,乙酸乙酯提取氯霉素可以有效降低它族抗生素的干扰。发光细菌法检测南美白对虾的方法检出限为0.16μg/kg,低于气相色谱的0.30μg/kg。Five luminescent bacteria strains （named DB,YL,BA,GQ,GX） were screened to detect the chloramphenicol in the aquatic foods. The results of sequences of 16SrDNA and physiological test showed that the five strains belong to Photobacterium phosphoreum, Photobacterium leiognathi, Vibrio harveyi, Vibrio splendidus and Vibrio fischeri respectively. The luminescence of YL （P. leiognathi） was stable and the max-luminescence lasted for about 16hrs. Antibiotic test results showed that YL （P. leiognathi） was most sensitive to chloramphenicol among the five strains. Compared with the GC, the sample preparation of luminescent bacteria method was simple, rapid and economical. Using luminescent bacteria YL to detect the chloramphenicol in spiked shrimp, the detection limit of 0.16μg/kg was reached, which was lower than 0.30μg/kg by GC.

关 键 词：氯霉素 鳆发光杆菌 发光细菌 16SrDNA 

分 类 号：TP274[自动化与计算机技术—检测技术与自动化装置]