菟丝子属5个种的种子DNA微量提取方法比较  

The comparison of four methods on DNA microextraction of five related species in Cuscuta.

作　　者：庄蓉[1] 郭琼霞[2] 黄可辉[2] 虞赟[2] 赵士熙[1] 黄娴[1] 

机构地区：[1]福建农林大学,福州350003 [2]福建出入境检验检疫局,福州

出　　处：《植物检疫》2008年第4期216-219,共4页Plant Quarantine

基　　金：福建科技重大专项专题(2006NZ0002-1);国家“十一五”重大科技支撑计划(2006BAD08A13)

摘　　要：本研究以Cuscuta属中5个种的种子为实验材料,分别采用CTAB、SDS、Moller与TaKaRa DNA提取试剂盒这4种方法提取DNA;并对不同方法所提取DNA的纯度、DNA质量浓度、DNA提取率进行比较分析。结果表明:用SDS的方法对Cuscuta属5个种的种子DNA的提取为最佳方法。DNA in five related species weeds of Cuscuta was isolated to use four methods including CTAB, SDS, Moiler and TaKaRa DNA extraction kit. The purity, concentration and extraction ratios of DNA in the prod- ucts obtained by different methods was analyzed and compared. Results showed that SDS was the best method to extract the genomic DNA in weeds of the five species.

关 键 词：菟丝子属 杂草种子 DNA提取方法 

分 类 号：S41[农业科学—植物保护] S451