氯苄基四氢小檗碱对大鼠肥厚心肌中KATP通道表达的影响  

The Expression of ATP-Sensitive Potassium(KATP) Channel in Infarct Produced Myocardial Remodeling of Rats

作　　者：刘青[1] 戴德哉[2] 

机构地区：[1]华侨大学材料科学与工程学院,福建泉州362021 [2]中国药科大学药理研究室,江苏南京210009

出　　处：《华侨大学学报（自然科学版）》2008年第3期395-398,共4页Journal of Huaqiao University(Natural Science)

基　　金：国家自然科学基金重点资助项目(30230170);华侨大学科研启动基金资助项目(05BS301)

摘　　要：观察心梗诱导的大鼠肥厚心肌中三磷酸腺苷(ATP)敏感性钾离子通道(KATP)的表达,并探讨其影响因素.将雄性大鼠随机分为5组,分别是假手术组,手术组,普萘洛尔组,氯苄基四氢小檗碱组及内皮素受体拮抗剂组.手术组及药物组的大鼠结扎冠脉左前降支,术后饲养10 d,药物组于术后第6天开始给药,共给药5d,由反转录-多聚酶链式反应(RT-PCR)方法确定KATP通道的mRNA表达.实验结果表明,心梗诱导的大鼠肥大心肌中的KATP通道的mRNA表达明显上调,经药物干预后有不同程度的降低.心梗诱导的大鼠肥厚心肌中的KATP通道的mRNA的表达上调,其表达上调除与β受体有关外,Ca2+离子可能参与对KATP通道表达的影响.Aim to observe the expression of ATP-sensitive potassium （KATe） channel in infarct produced myocardial remodeling and the factors were studied. Methods： male sprague-dawley rats randomly divided into five groups, sham-operated, operated and three treatment groups including propranolol, p-chlorobenzyltetrahydroberberine and endothelin receptor antagonist. Myocardial infarction was induced by the left anterior descending coronary artery was ligated with a silk suture. Sham-operated controls were treated likewise, except that the suture around the coronary artery was not closed. From 5 d the treatment groups were administered drugs after coronary artery ligation. All animals were killed on 10 d after ligation. The expression of mRNA for KAXP channels was studied in rat hypertrophic myocardium using reverse transcription-polymeric chain reaction （RT-PCR）. Results： The expression of KATe channel in infarct produced myocardial remodeling displayed up-regulted. After treatment the expression of KATe channel shown down-regulated in different degree. Conclusion： The expression of KATe channel in infarct produced myocardial remodeling displayed up-regulated. The factors involved in Ca^2＋ except β receptor, the effect of Ca^2＋ on the expression of KATe in infarct produced myocardial need study more.

关 键 词：心肌肥厚 心梗 ATP敏感性钾离子通道 反转录-多聚酶链式反应 

分 类 号：R972[医药卫生—药品] R-332[医药卫生—药学]