克伦特罗流式微球竞争性免疫检测技术及试剂  

A competitive immunoassay and reagents using flowing fluorescent-code microspheres for screening of clenbuterol

作　　者：高海霞[1] 邹明强[1] 王岭[1] 李锦丰[1] 蒋继志[2] 崔霞[1] 胡秀丽[1] 陈立本[1] 

机构地区：[1]中国检验检疫科学研究院,北京100025 [2]河北大学生命科学学院,河北保定071002

出　　处：《化学试剂》2008年第7期509-511,共3页Chemical Reagents

摘　　要：以荧光编码微球为免疫反应载体，通过流式细胞仪的荧光信号检测，对试剂加入顺序、加入量、反应时间进行优选，建立流式微球免疫检测克伦特罗的新方法。该方法灵敏度高，检测限0．01ng／mL，特异性强，实测猪尿中克伦特罗残留含量，加标回收率为96％～119％，变异系数在6．5％～15．3％之间，结果令人满意。A novel competitive immunoassay by flow cytometry to rapidly screen clenbuterol in samples using fluorescent-code beads as the carrier was established. The optimized test conditions were focused on reagent adding order, reagent concentration, and reaction time. The method was proved to be sensitive, specific, accurate and stable, with such satisfactory results as a detection limit of 0.01 ppb,a recovery in the range of 96% - 119% and a coefficient of variation ranging from 6.5% to 15.3% for determination of clenbuterol in pig urines.

关 键 词：流式微球 试剂 竞争性免疫检测 克伦特罗 

分 类 号：O657[理学—分析化学] S482[理学—化学]