反相高效液相色谱法测定吲达帕胺的含量  

Determination of indapamide by RP-HPLC

作　　者：祝艳琳[1] 章靖[1] 田丹碧[1] 

机构地区：[1]南京工业大学理学院,江苏南京210009

出　　处：《化学试剂》2008年第7期512-514,共3页Chemical Reagents

摘　　要：建立RP-HPLC法测定吲达帕胺含量的新方法。采用HPLC法，色谱柱为Kromasil C18柱；流动相中加入新的流动相-乙腈，V（水）：V（乙腈）：V（甲醇）：V（冰醋酸）=65：17．5：17．5：O．1，流速1．0mL／min，检测波长240nm。该方法的线性范围为52．0—260．0μg／mL（r=O．9994），RSD=0．14％（n=6）。本法简便、准确、灵敏、重现性好，适合吲达帕胺片的质量控制。A new RP-HPLC method for the determination of indapamide was established. A Kromasil C18 column was used with the mobile phase composed of water-acetonltrile-methanol-glacial acetic acid（at a ratio of 65 ： 17.5 ： 17.5.0.1 ）. The flow rate was 1.0 mL/min and the detection wavelength was 240 nm. The calibration curve of indapamide was linear in a concentration range of 52.0～260.0 μg/mL （r =0.999 4）and the average recovery （RSD） was 0.14% （ n = 6）. This method was simple, accurate, sensitive and reproducible, and could be used for quality control of indapamide tablet.

关 键 词：高效液相色谱 吲达帕胺 含量测定 

分 类 号：O657.7[理学—分析化学]