茚虫威在直链淀粉手性固定相上的对映体分离研究  

Enantiomeric separation of indoxacarb by HPLC with amylose chiral column

作　　者：董丰收[1,2] 曹巧[2] 刘新刚[2] 郑永权[2] 李重九[1] 

机构地区：[1]中国农业大学理学院,北京100094 [2]中国农业科学院植物保护研究所,农业部农药化学与应用技术重点开放实验室,北京100094

出　　处：《化学试剂》2008年第7期517-518,521,共3页Chemical Reagents

基　　金：国家重点基础研究973项目(2002CB410806)

摘　　要：茚虫威对映异构体在三[（S）-a-甲基苯基氨基甲酸]直链淀粉酯涂敷型高效液相色谱手性固定相上成功的获得了拆分。通过考察流动相中醇类改性剂种类和浓度对其对映体分离的影响，优化了色谱分离条件。结果显示：随着流动相中异丙醇和乙醇改性剂含量增加，分离度逐渐变小，当异丙醇和乙醇的含量分别为20％时，茚虫威即可达到基线分离，分离度为1．8和1．25；两种改性剂比较，乙醇比异丙醇有更高的改性效率。同时考察了分离温度对茚虫威手性分离的影响，结果表明，在15～35℃范围内，温度升高和分离度增大呈正相关关系。Enantiomeric separation of indoxacarb was performed by high performance liquid chromatographywith the coating-type amylose-itris [（ S ）-a-methyphenylcarbamate] used as the chiral stationary phase （CSP）. The chromatographic conditions were optimized by studying the influences of the mobile phase composition including the concentration and type of the alcohol modifiers in bexane on enantiomers separation. The data indicated that the resolution of indoxacarb enantiomers decreased with the modifier contents in hexane increased, and indoxacarb could achieve the baseline separation with a resolution of 1.8 and 1.25 at a con- centration of 20% iso-propanol or ethanol in the mobile phase. Correspondingly, as an alcohol modifier, ethanol had a better modifier effect than isopropanol. The effect of temperature on chiral separation was investigated and it showed that a higher column temperature resulted in a higher separation efficiency for indoxacarb in the temperature range of 15～35 ℃.

关 键 词：茚虫威 三[(S)-a-甲基苯基氨基甲酸]直链淀粉酯 手性拆分 手性固定相 

分 类 号：O658[理学—分析化学]