红树林共生真菌Paecilomyces sp. Tree 1-7代谢产物对人肝癌细胞HepG2的杀伤作用  

Cytotoxicity of the Secondary Metabolites of Marine Mangrove Fungus Paecilomyces sp. Tree 1-7 on Human Hepatoma Cell Line HepG2

作　　者：蔡小玲[1] 高俊平[1] 李庆[1] 温露[1] 佘志刚[1] 林永成[1] 

机构地区：[1]中山大学化学与化学工程学院,广东广州510275

出　　处：《中药材》2008年第6期864-865,共2页Journal of Chinese Medicinal Materials

基　　金：国家高科技发展(863)资助项目(2006AA09Z422);国家自然科学基金项目(20572136);广东省自然科学基金项目(5300528);中山大学青年教师基金资助项目(2005310001131133)

摘　　要：目的:测定红树林共生真菌Paecilomycessp.Tree 1-7代谢产物对人肝癌细胞HepG2的杀伤作用。方法:实验分为3组:空白对照组、阳性对照组和代谢产物组,采用MTT法检测不同浓度的代谢产物对人肝癌细胞HepG2的杀伤作用。结果:代谢产物中化合物secalonic acid A对人肝癌细胞HepG2的IC50为2.0μg/ml,tenellic acid A对HepG2的IC50为62.1μg/ml,alternin对HepG2的IC50为7.0μg/ml。结论:红树林共生真菌Paecilomycessp.Tree 1-7代谢产物中secalonic acid A和alternin对人肝癌细胞HepG2具有较强的细胞毒性,值得进一步研究。Objective ： To study the cytotoxicity of the seeondary metabolites of Marine Mangrove Fungus Paecilomyces sp. Tree 1- 7 on human hepatoma cell line HepG2 cultured in vitro. Methods：Three groups were divided：compounds group,5-Fu group and control group. The cytotoxicity was measured by MTT method when HepG2 cells were treated by different concentration of the secondary metabolites of Paecilorayces sp. Tree 1-7. Results：Secalonic acid A, tenellic acid A and alternin inhibited the growth of human hepatoma cell line HepG2,the IC50 separately were 2.0,62. 1 and 7. 0 μg/ml. Conclusion：Secalonic acid A and ahernin have strong cytotoxicity on HepG2 cultured in vitro.

关 键 词：红树林共生菌 人肝癌细胞HEPG2 细胞毒性 

分 类 号：R285.5[医药卫生—中药学]