Fas和caspase-8信号通路在外周听觉系统老化中的作用和机制研究  

The Roles and Mechanism of Fas and caspase-8 Signal Transduction Pathway in Cochlea in Response to Aging

作　　者：罗凌惠[1] 龚树生[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院耳鼻咽喉科,武汉430022 [2]首都医科大学附属北京同仁医院耳鼻咽喉-头颈外科

出　　处：《听力学及言语疾病杂志》2008年第4期313-316,共4页Journal of Audiology and Speech Pathology

基　　金：国家自然科学基金资助项目(30471879);高校青年教师奖;湖北省卫生厅科研基金资助项目(JXIBO56)

摘　　要：目的检测老年大鼠耳蜗Fas受体及caspase-8mRNA表达水平变化，研究细胞死亡受体途径在外周听觉系统老化中的作用和机制。方法以老年Wistar大白鼠（20～22月龄）及成年Wistar大白鼠（2～3月龄）各10只为实验对象，采用半定量逆转录聚合酶链反应（reverse transcription polymerasechain reaction．RT-PCR）的方法检测耳蜗组织Fas受体及caspase-8基因mRNA的含量。结果老年组耳蜗组织Fas受体及caspase-8mRNA的表达水平均高于成年组，差异均有显著统计学意义（P〈0．01）。结论老年大鼠耳蜗Fas及caspase8表达明显升高，说明耳蜗的老化与fas途径的凋亡密切相关；Fas及caspase-8信号通路增强可能在老年性聋发病机制中有重要作用。Objective To determine the changes of the mRNA expression of Fas and caspase-8 genes in response to aging. Methods The expression of Fas and caspase-8 mRNA was detected in the cochlea of the aged and adult rats by a semi-quantitative reverse transcription polymerase chain reaction method. Results Both Fas and easpase 8 mRNA levels in tbe cochlea of the aged animals were significantly higher than those in adult ones（P〈0. 01 ）. Conclusion The increased expression levels of Fas and caspase 8 in aged rats might make the aging cochlea more susceptible to damage. The results suggested that aging in cochlea might have close correlation with fas receptor mediated apoptosis. The increased fas and caspase-8 signal transduction pathway might play an important role in pathogenic mechanism of presbycusis.

关 键 词：FAS受体 Caspase-8基因 耳蜗 老化 半定量逆转录聚合酶链反应 

分 类 号：R764.436[医药卫生—耳鼻咽喉科]