LOX-1介导苯扎贝特对内皮细胞凋亡的影响  

Lectin-like oxidized low density lipoprotein receptor-1 mediates effects of bezafibrate on the apoptosis of endothelial cells

作　　者：中晓彧[1] 薛丽霞[1] 屈巧芳[1] 曾秋棠[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院心内科,湖北武汉430022

出　　处：《中国医师杂志》2008年第6期754-757,共4页Journal of Chinese Physician

基　　金：山西省太原市科技局科技兴市基金项目（0703046）

摘　　要：目的研究苯扎贝特对氧化型低密度脂蛋白（ox—LDL）诱导人脐静脉内皮细胞（HUVECs）凋亡的影响及血凝素样氧化型低密度脂蛋白受体1（LOX-1）在其中的作用。方法用流式细胞仪观察HUVECs凋亡，用RT-PCR技术观察LOX-1基因表达的变化。结果ox—LDL组与正常组比较凋亡明显增加（P〈0．05），LOX-1 mRNA表达明显增加（P〈0．05），苯扎贝特干预后凋亡减少，LOX-1 mRNA的表达降低（P〈0．05），呈浓度依赖关系。用LOX-1阻滞剂多聚肌苷酸预先干预2h后与苯扎贝特组比较细胞凋亡及LOX-1mRNA表达进一步减少（P〈0．05）。结论苯扎贝特可改善ox-LDL对内皮细胞的影响，下调LOX-1表达，减少内皮细胞凋亡，部分机制可能通过LOX-1介导。Objective To observe the influence of bezafibrate on the apoptosis and expression of lectin-like oxidized low density lipoprotein receptor-1 （LOX-1） mediated by oxidized low density lipoprotein（ox-LDL） in cultured human umbilical vein endothelial cells（ HUVECs）. Methodes The apoptosis of HUVECs mediated by ox-LDL were evaluated by flow cytometry and the expression of LOX-1 mRNA were detected by RT-PCR. Results Compared with the control group, ox-LDL could increase apoptosis and the expression of LOX-1 （ P 〈 0. 05）, and Bezafibrate could decrease the apoptosis and the expression of LOX-1 in a concentration -dependent manner（ P 〈 0. 05）. Preincubation of HUVECs with polyinosinic acid for 2 hours, the apoptosis and the expression of LOX-1 decreased（ P 〈 0. 05）. Conclusions Bezafibrate inhibits the apoptosis of HUVECs mediated by ox-LDL by reducing the expression of LOX-1, which may be part of the reasons for bezafibrate to prevent and treat atherosclerosis.

关 键 词：苯扎贝特/药理学 受体 LDL 细胞凋亡 

分 类 号：R543.5[医药卫生—心血管疾病] R322.12[医药卫生—内科学]