一氧化氮在异氟烷预处理脑保护中基因表达的作用  

The effect of nitric oxide on expression of isoflurane preconditioning against brain ischemia-reperfusion

作　　者：沈洁[1] 陈卫民[1] 

机构地区：[1]中国医科大学附属盛京医院麻醉科,辽宁沈阳110004

出　　处：《中国医师杂志》2008年第6期767-770,共4页Journal of Chinese Physician

摘　　要：目的通过观察异氟烷预处理在鼠脑缺血再灌注损伤中凋亡相关基因Bcl-2和Bax的mRNA的表达，探讨异氟烷预处理脑保护作用的一氧化氮信号传导通路。方法75只雄性沙士鼠，随机分5组。假手术组（SHAM组）：只分离双侧颈总动脉而未子夹闭；缺血再灌注组（I／R组）：夹闭双侧颈总动脉5min后开放造成I／R；异氟烷预处理组（ISO组）：I／R前60min吸入1．2％～1．5％异氟烷30min；氨基呱（AG）＋ISO组：腹腔内注射AG200mg／kg，30min后同ISO组；AG组：I／R前30min注射AG。再灌注24h．取鼠前脑，采用RT-PCR检测脑组织中Bcl-2、Bax基因的mRNA表达。结果ISO组Bcl-2的mRNA表达增高（1．22±0．12）．与SHAM组（0．83±0．16）、I／R组（0．83±0．11）和AG＋ISO组（0．82±0．12）比较差异有统计学意义（P〈0．05）；I／R和AG组BaxmRNA的相对表达量明显增高，与SHAM组比较差异有统计学意义（P〈0．05），ISO组和AG＋ISO组表达较弱，但AG＋ISO组比ISO组表达略强。结论一氧化氮信号传导通路是异氟烷预处理脑保护作用的途径之一。Objective To investigate the effect of isoflurane preconditioning on Bcl-2 and Bax mRNA expression and the signaling pathway of nitric oxide against brain ischemia-reperfusion （I/R）. Methods Seventy-five male gerbils were randomly divided into 5 groups （ n = 15） ： SHAM, I/R, ISO, AG ＋ ISO and AG group. Global cerebral I/R was produced by occlusion of bilateral common carotid arteries for 5 minutes and confirmed by isoelectric potential on EEG. In ISO group, the animals inhaled 1.2% - 1.5% isoflurane for 30 minutes followed by 30 minutes wash-out period before I/R. In AG ＋ ISO group, 30 minutes after aminoguanidine, 200 mg/kg i. p. isoflurane was inhaled as ISO group, and aminognanidine was injected 30 min before I/R in AG group. After 24 hours, the forebrains were collected, and the expression of Bcl-2 and Bax mRNA was determined by reverse transcriptase polymerase chain reaction. Results Bcl-2 mRNA expression in ISO group（ 1.22 ±0. 12 ） was significantly higher than that in sham（0. 83 ±0. 16）, I/R （0. 83 ±0. 11 ）and AG ＋ ISO group（0. 82 ±0. 12） （ P 〈 0.05 ）. Bax mRNA expression in I/R and AG group was significantly higher than that in sham group （ P 〈 0.05 ）, and the expression in ISO group was higher than that in AG ＋ ISO group. Conclusion Nitric oxide is involved in the signaling pathway of isoflurane preconditioning and has the protection effect on gerbil brain against I/R.

关 键 词：一氧化氮 异氟彰药理学 再灌注损伤 脑缺血 基因表达 

分 类 号：R96[医药卫生—药理学]