Nell-1基因转染骨髓基质干细胞复合可吸收纤维蛋白胶修复犬下颌骨缺损  

Nell-1 transferred BMSC combined with absorbable fibrin glue repairs mandible defect in dogs

作　　者：张宇[1] 陈明[2] 高杰[1] 

机构地区：[1]南方医科大学南方医院口腔科,广州510515 [2]解放军总医院第二附属医院口腔科,北京100091

出　　处：《第三军医大学学报》2008年第14期1327-1329,共3页Journal of Third Military Medical University

基　　金：全军医学科研“十一五”计划面上项目(06MA1134)~~

摘　　要：目的探讨利用Nell-1基因转染骨髓基质干细胞(bone marrow stromal cell,BMSC)复合可吸收纤维蛋白胶(fibrin glue,FG)构建组织工程化骨组织并修复犬下颌骨缺损的可行性。方法制备含Nell-1真核细胞表达载体的逆转录病毒液PT-PLNCX2-Nell-1并转染BMSC,使用免疫组织化学的方法检测Nell-1在BMSC中的表达。犬下颌骨缺损修复实验分为4组:转染组、未转染组、单纯FG组和对照组,每组4个样本。分别于术后8、16周采用大体观察、X线、组织学检测等方法对骨缺损修复情况进行对比。结果免疫组织化学检测显示经Nell-1病毒液转染的BMSC中有较强的阳性结果出现。定量检测分析结果证实经转染的BMSC修复骨缺损的成骨速度和成骨量均优于未转染组,单纯FG组和对照组中均未见骨缺损得到修复。结论Nell-1基因转染能够提高BMSC形成组织工程化骨组织和修复骨缺损的能力,可用于以BMSC为种子细胞的组织工程化骨组织的构建。Objective To explore the effect of bone marrow stromal cells （BMSC） transferred by Nell-1 gene combined with biomaterial fibrin glue （FG） to enhance segmental bone defect healing in dog mandible. Methods Nell-1 gene vector was reconstructed in retroviral vector and then transfected BMSC. The protein of Nell-1 gene in transferred cells was determined by immunohistochemistry. Segmental defects were created surgi- cally in the dog＇ s mandible. The defect was repaired with BMSC transfected by Nell-1 retroviral granules in presence of FG, untransfected BMSC in combination with FG, and FG alone. The control group was left un- treated. The defect-repairing capability for each treatment were assessed by gross observation, radiography, and histology at 8th week and 16th week. Results Cells transfected by Nell-1 retroviral granules expressed abundant Nell-1 mRNA and protein in the cytoplasm. Positive results were not found in those cells that were not transferred. The use of BMSC transferred by Nell-1 retroviral granules combined with FG materials exhibited the strongest defect-repairing ability. Gross observation, radiographical and histomorphometric analyses revealed a significantly greater total area of bone formation, increased amount of the new bone in the defects than in those treated with the untransfected BMSC. Conclusion Nell-1 gene transfection may be used to promote the osteogenie ability of BMSC.

关 键 词：Nell-1 骨缺损 基因转染 

分 类 号：R318.08[医药卫生—生物医学工程] R329.21[医药卫生—基础医学]