乙肝多表位抗原基因非融合表达载体的构建和表达  

Construction and expression of non-fusional expression vector of the multi-epitope gene of hepatitis B virus

作　　者：骆利敏[1] 夏虎[2] 李灼亮[1] 余宙耀[1] 刘树人[1] 李明[3] 

机构地区：[1]解放军第458医院肝病中心传染科 [2]南方医科大学附属珠江医院 [3]南方医科大学分子生物学院,广东广州510602

出　　处：《细胞与分子免疫学杂志》2008年第8期788-790,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30170532);广东省自然科学基金资助项目(990375);广州市重点攻关课题资助(2001-2-035-01)

摘　　要：目的:研究乙型肝炎病毒(HBV)多表位基因的在原核载体中的克隆、游离表达及其产物的抗原性。方法:设计、并合成HBV多表位抗原基因BPT,克隆入原核表达载体pBAD/gIIIA,进而转化Top10大肠杆菌,阿拉伯糖诱导表达出HBV多表位抗原蛋白(B-BPT),并用Western blot方法初步检测该抗原蛋白的抗原性。结果:成功构建了原核表达载体pBAD/BPT,在大肠杆菌中表达出HBV多表位抗原蛋白B-BPT,Western blot检测显示该蛋白具有良好抗原性。结论:HBV多表位抗原基因的设计是成功的,其在大肠杆菌中表达的非融合蛋白具有良好的抗原性,可能是较理想的HBV治疗性疫苗候选物。AIM： To study the cloning,expression and antigen of therapeutic multi-epitope gene of hepatitis B virus.METHODS： The multi-epitope gene of hepatitis B virus（BPT） was designed,synthesized and cloned into the prokaryotic expression vector pBAD/gIIIA.Then it was transformed into E.coli Top10 and multi-epitope protein of hepatitis B virus（B-BPT） was expressed under the induction of Arabinose.The immunogenicity of the protein was analyzed by Western blot detection.RESULTS： The recombinant plasmid pBAD/BPT was constructed successfully and the protein of multi-epitope gene of hepatitis B virus was expressed in E.coli.Western blot detection showed the protein had ideal antigenicity.CONCLUSION： The design of therapeutic multi-epitope gene of hepatitis B virus is proved to be correct.The expressed protein may be a good therapeutic vaccine.

关 键 词：乙型肝炎病毒 多表位 治疗性疫苗 

分 类 号：Q78[生物学—分子生物学] R373.2[医药卫生—病原生物学]