TNFSF14及HVEM在自身免疫性心肌炎中的表达及意义  

Expression and Significance of TNFSF14 and HVEM in the Experimental Autoimmune Myocarditis

作　　者：彭晟[1] 唐其柱[1] 郑茜[1] 沈涤非[1] 初洪刚[2] 郭海鹏[1] 

机构地区：[1]武汉大学人民医院心内科,湖北武汉430060 [2]武汉大学人民医院超声影像科,湖北武汉430060

出　　处：《武汉大学学报（医学版）》2008年第4期458-462,I0002,共6页Medical Journal of Wuhan University

基　　金：武汉市科技计划项目(编号:20056001021-02)

摘　　要：目的:建立自身免疫性心肌炎大鼠模型,探讨肿瘤坏死因子超家族成员(TNFSF14)LIGHT及其受体HVEM在实验性自身免疫性心肌炎大鼠心肌组织表达及意义。方法:从猪心室肌组织提取心肌肌球蛋白,以猪心肌肌球蛋白与完全弗氏佐剂混合的乳浊液间隔7 d皮下注射免疫模型组大鼠,对照组大鼠仅用完全弗氏佐剂皮下注射。分别在初次免疫后第21天和第90天检测超声心动图,取心肌组织作病理学检查,用RT-PCR法检测心肌组织LIGHT及HVEM mRNA的表达。结果:在急性期(第21天),模型组大鼠心肌组织HE染色见有不同程度的急性炎性细胞浸润和心肌细胞变性坏死,同时超声心动图检测到心功能的下降。第90天,模型组大鼠心肌组织炎症减弱,并伴有纤维化出现,对照组均未出现心肌炎症和纤维化。在第21天,模型组大鼠心肌组织中LIGHT和HVEM基因表达水平均高于对照组(P<0.01)。第90天,模型组LIGHT与HVEM基因表达仍然高于对照组(P<0.01)。结论:LIGHT及HVEM基因在实验性自身免疫性心肌炎大鼠心肌中的表达是上调的,LIGHT/HVEM通路可能参与自身免疫性心肌炎发生发展过程。Objective： To establish animal model of experimental autoimmune myocarditis in Lewis rats and investigate the expression of LIGHT（TNFSF14） and HVEM in the experimental autoimmune rnyocarditis and its significance. Methods： Cardiac myosin was extracted from porcine ventricular myocardium. Genetically predisposed Lewis rats were immunized with cardiac myosin covered by complete Freund＇s adjuvant （CFA） on day 0 and 7 to establish the experiment autoimrnune rnyocarditis（EAM） models. The control rats were immunized with CFA only. On day 21 and 90 after the first immunization, echocardiography was examined, histopathological changes of rnyocardium were detected by HE pigmentation, and gene levels of LIGHT and HVEM was measured by semi-quantitative RT-PCR. Results： In model rats, histopathological examination of cardiac tissue showed an obvious inflammatory cell infiltration with myocytes necrosis on day 21,simultaneously, echocardiography examination showed that heart function decreased as compared with control group（P〈0.05）. On day 90, fibrosis and a few of inflammatory cells could be observed. No inflammation and fibrosis was emerged in the myocardium of control rats. In addi tion, as compared with that in control groups, mRNA abundance for both LIGHT and HVEM were up regulated （P〈0.05） at acute phase （day 21） in the EAM models. On day 90, the mRNA abundance for LIGHT and HVEM was still up regulated （P〈0.05）. Conclusion： The ex pression of LIGHT and HVEM mRNA in myocardium was up-regulated in experimental autoimmune myocarditis. The LIGHT/HVEM pathway maybe plays a role in the occurrence and development of autoimmune myocarditis.

关 键 词：心肌肌球蛋白 自身免疫性心肌炎 TNFSF14 HVEM 

分 类 号：R542.21[医药卫生—心血管疾病]