1株鼠源狂犬病毒野毒株全基因组序列测定与分析  

Sequencing and analysis of the complete genome of one wild strain of vole rabies virus

作　　者：赵云蛟[1] 郭利[1] 黄莹[1] 张立世[1] 钱爱东[1] 

机构地区：[1]吉林农业大学动物科学技术学院,长春130118

出　　处：《中国人兽共患病学报》2008年第7期651-654,658,共5页Chinese Journal of Zoonoses

基　　金：国家自然科学基金资助项目(30070542)

摘　　要：目的对1株分离自牛狂犬病疫区野鼠脑内的狂犬病毒野毒株(MRV)进行全基因组测序,并对其分子变异和进化特点进行分析。方法RT-PCR扩增克隆覆盖全基因组9个重叠基因片段,基因组3′末端和5′末端采取3′-RACE和5′-RACE方法。结果9个重叠基因片段序列拼接得到MRV全基因组序列,共11869个核苷酸。MRV毒株全基因组构成与其他狂犬病毒基因组构成相似,由5个编码区组成,基因起始位点和终止位点高度保守,在核蛋白和糖蛋白的重要抗原位点有个别氨基酸发生变异。选择有代表性的狂犬病毒株,构建了N基因分子系统进化树。结论MRV毒株属于狂犬病毒基因1型,与AV01和CVS同源性最高为99%,与分类位置未确定的北高加索毒株(WCBV)的同源性最低为72%。The complete genome of one wild strain of vole rabies virus was sequenced and its molecular variations and evolutionary property were analyzed,in which 9 overlapped gene fragments were amplified by RT-PCR through the 3＇-RACE and 5＇-RACE method. It was found that the complete genome sequence of 9 overlapped gene fragments in vole rabies virus with 11869 nucleotides could be obtained. The structural components of the genome was quite similar to those of other rabies viruses,consisting of 5 genes,the start codon and the stop codon which were highly conserved. Some individual amino acid variations could be detected in the important loci of nucleoproteins and glycoprotein. By comparing with the N gene sequences of other typical rabies viruses, the phylogenetic tree could be established. This strain of virus should belong to the type 1 of rabies virus having a homology of 99% with AV01 and CVS strains and a homology of 72% with the unclassified WCBV strain.

关 键 词：狂犬病毒 全基因组 序列分析 

分 类 号：R373.9[医药卫生—病原生物学]